20-羟基蜕皮酮诱导果蝇Kc细胞中一个β-微管蛋白基因的表达。

20-Hydroxyecdysone induces the expression of one beta-tubulin gene in Drosophila Kc cells.

作者信息

Montpied P, Sobrier M L, Chapel S, Couderc J L, Dastugue B

机构信息

Laboratoire de Biochimie Médicale, U.A. C.N.R.S. 360, Faculté de Médecine, Clermont-Ferrand, France.

出版信息

Biochim Biophys Acta. 1988 Jan 25;949(1):79-86. doi: 10.1016/0167-4781(88)90057-7.

DOI:10.1016/0167-4781(88)90057-7

PMID:2825814

Abstract

The expression of 56D and 60C beta-tubulin genes has been examined in Drosophila melanogaster Kc cells in response to the insect moulting hormone, 20-hydroxyecdysone (20-OH-E). Northern blots probed with beta-tubulin subclones show that the 56D beta-tubulin gene encodes a 1.8 kb mRNA whose abundance is not affected by 20-OH-E. The 60C gene probe detects two mRNAs: one of 1.8 kb present in untreated and 20-OH-E-treated cells, and one of 2.6 kb present only in 20-OH-E-treated cells; using a 60C 3'-specific probe, only the 2.6 kb is revealed. Hybrid selection translation experiment demonstrates that a 20-OH-E-inducible mRNA homologous to the 60C gene encodes a beta-tubulin subunit (P4); this subunit is the so-called beta 3-tubulin. Translation of size-fractionated mRNA shows that the 20-OH-E-induced beta 3-tubulin subunit is encoded, in treated cells, by the 2.6 kb mRNA.

摘要

在黑腹果蝇Kc细胞中，研究了56D和60Cβ-微管蛋白基因对昆虫蜕皮激素20-羟基蜕皮酮（20-OH-E）的响应表达。用β-微管蛋白亚克隆探针进行的Northern印迹显示，56Dβ-微管蛋白基因编码一种1.8 kb的mRNA，其丰度不受20-OH-E的影响。60C基因探针检测到两种mRNA：一种1.8 kb的存在于未处理和20-OH-E处理的细胞中，另一种2.6 kb的仅存在于20-OH-E处理的细胞中；使用60C 3'-特异性探针，仅显示出2.6 kb的mRNA。杂交选择翻译实验表明，与60C基因同源的一种20-OH-E诱导型mRNA编码一种β-微管蛋白亚基（P4）；该亚基即所谓的β3-微管蛋白。大小分级mRNA的翻译表明，在处理过的细胞中，20-OH-E诱导的β3-微管蛋白亚基由2.6 kb的mRNA编码。