Sobrier M L, Couderc J L, Chapel S, Dastugue B
Biochem Biophys Res Commun. 1986 Jan 14;134(1):191-200. doi: 10.1016/0006-291x(86)90546-2.
One polypeptide, P4, of molecular weight 55000 daltons and pHi, 5,1, is synthesized in Drosophila melanogaster Kc 0% cells only when they were treated by the insect moulting hormone, 20 hydroxyecdysone (20-HE). P4 is precipitated with vinblastine sulfate and migrates as a tubulin subunit. Immunoblot experiment confirms that P4 is a beta subunit of tubulin. This beta tubulin is recovered by in vitro translation only when mRNAs are extracted from treated cells. Thus, regulation of expression of this polypeptide is at the level of transcripts and is under a steroid hormone control. The 20-HE induced beta tubulin comigrates with the beta 3 subunit and like the latter, is specific to the mid period of embryogenesis.
一种分子量为55000道尔顿、pHi为5.1的多肽P4，仅在黑腹果蝇Kc 0%细胞受到昆虫蜕皮激素20-羟基蜕皮酮（20-HE）处理时才会合成。P4能与硫酸长春碱沉淀，并作为微管蛋白亚基迁移。免疫印迹实验证实P4是微管蛋白的β亚基。只有从处理过的细胞中提取mRNA时，才能通过体外翻译回收这种β微管蛋白。因此，这种多肽的表达调控发生在转录水平，且受类固醇激素控制。20-HE诱导的β微管蛋白与β3亚基共迁移，并且与后者一样，在胚胎发育中期具有特异性。
