Lokesh B R, German B, Kinsella J E
Lipid Research Laboratory, Institute of Food Science, Cornell University, Ithaca, NY 14853.
Biochim Biophys Acta. 1988 Jan 19;958(1):99-107. doi: 10.1016/0005-2760(88)90250-0.
Docosahexaenoic acid (DHA) or eicosapentaenoic acid (EPA) was facilely incorporated into phospholipids of mouse peritoneal macrophages following incubation with pure fatty acids complexed to bovine serum albumin. Following stimulation with calcium ionophore A23187, the DHA-enriched cells synthesized significantly smaller amounts of leukotriene C4 and leukotriene B4 compared to control or EPA-enriched cells. The EPA-enriched cells synthesized lower amounts of leukotriene C4 and leukotriene B4 compared to control cells. The stimulated macrophages utilized endogenously released arachidonic acid for leukotriene B4 and leukotriene C4 synthesis. Exogenous arachidonic acid increased the formation of 12-hydroxyeicosatetraenoic acid (12-HETE) and 15-HETE and macrophages enriched with DHA or EPA produced similar amounts of 12-HETE and 15-HETE compared to control cells. These studies demonstrated that the synthesis of leukotriene C4, leukotriene B4 and HETE in macrophages is differentially affected by DHA and EPA.
将与牛血清白蛋白复合的纯脂肪酸孵育后，二十二碳六烯酸（DHA）或二十碳五烯酸（EPA）很容易掺入小鼠腹膜巨噬细胞的磷脂中。在用钙离子载体A23187刺激后，与对照细胞或富含EPA的细胞相比，富含DHA的细胞合成的白三烯C4和白三烯B4的量显著减少。与对照细胞相比，富含EPA的细胞合成的白三烯C4和白三烯B4的量较低。受刺激的巨噬细胞利用内源性释放的花生四烯酸来合成白三烯B4和白三烯C4。外源性花生四烯酸增加了12-羟基二十碳四烯酸（12-HETE）和15-HETE的形成，与对照细胞相比，富含DHA或EPA的巨噬细胞产生的12-HETE和15-HETE的量相似。这些研究表明，巨噬细胞中白三烯C4、白三烯B4和HETE的合成受到DHA和EPA的不同影响。
