de Souza Thamires Klein, Soares Scheila Silva, Benitez Lisianne Brittes, Rott Marilise Brittes
Departamento de Microbiologia, Imunologia e Parasitologia, Instituto de Ciências Básicas da Saúde, Setor de Parasitologia, Universidade Federal do Rio Grande do Sul, Rua Sarmento Leite, N° 500, Porto Alegre, RS, Brazil.
Departamento de Biologia e Farmácia, Universidade de Santa Cruz do Sul, Avenida Independência, N° 2293, Santa Cruz do Sul, RS, Brazil.
Curr Microbiol. 2017 May;74(5):541-549. doi: 10.1007/s00284-017-1196-z. Epub 2017 Mar 3.
The interactions that occur between bacteria and amoebae can give through mutual relations, where both organisms benefit from the association or parasitic in which one organism benefits at the expense of the other. When these organisms share the same environment, it can result in some changes in the growth of organisms, in adaptation patterns, in morphology, development or even in their ability to synthesize proteins and other substances. In this study, the interaction between Acanthamoeba polyphaga and Staphylococcus aureus (MRSA) was evaluated using a co-culture model at different incubation times. The results showed that 89% of amoebic cells remained viable after contact with the bacteria. The bacterial isolate was visualized inside the amoeba through confocal microscopy and fluorescence for up to 216 h of co-cultivation. The lysate of amoebic culture increased the growth of S. aureus (MRSA), and the effect of supernatant of culture inhibited bacterial growth over the incubation times, suggesting that A. polyphaga produced some metabolite, that inhibited the growth of bacteria. Moreover, the encystment of the A. polyphaga was increased by the bacteria presence. The results show that A. polyphaga and S. aureus interaction may have an important influence on survival of both, and specially indicate a possible effect on the metabolics characteristics each other.
细菌与变形虫之间发生的相互作用可以通过互利关系实现，即两种生物都能从这种关联中受益，或者通过寄生关系，其中一种生物以牺牲另一种生物为代价而获益。当这些生物共享同一环境时，可能会导致生物体生长、适应模式、形态、发育甚至其合成蛋白质和其他物质的能力发生一些变化。在本研究中，使用共培养模型在不同孵育时间评估了多噬棘阿米巴与金黄色葡萄球菌（耐甲氧西林金黄色葡萄球菌）之间的相互作用。结果表明，89%的阿米巴细胞在与细菌接触后仍保持活力。通过共聚焦显微镜和荧光观察，在共培养长达216小时的时间内，细菌分离株可在变形虫内部观察到。阿米巴培养物的裂解物增加了金黄色葡萄球菌（耐甲氧西林金黄色葡萄球菌）的生长，而培养上清液在孵育期间抑制了细菌生长，这表明多噬棘阿米巴产生了一些抑制细菌生长的代谢物。此外，细菌的存在增加了多噬棘阿米巴的包囊形成。结果表明，多噬棘阿米巴与金黄色葡萄球菌的相互作用可能对两者的生存产生重要影响，特别表明可能对彼此的代谢特征产生影响。
