ANDE, 266 Second Avenue, Waltham, MA, 02451, USA.
Int J Legal Med. 2017 Nov;131(6):1489-1501. doi: 10.1007/s00414-017-1567-9. Epub 2017 Mar 3.
Rapid DNA identification is the use of a rugged, field-deployable system to generate short tandem repeat (STR) profiles in law enforcement, military, immigration, and homeland security applications. A performance verification study was conducted on the ANDE Rapid DNA identification system using FlexPlex27, a highly multiplexed, 27 locus assay that generates data for the expanded CODIS core loci and all additional STR loci required for international databasing. The assay contains 23 autosomal loci (D1S1656, D2S1338, D2S441, D3S1358, D5S81, D6S1043, D7S820, D8S1179, D10S1248, D12S391, D13S317, D16S539, D18S51, D19S433, D21S11, D22S1045, FGA, CSF1PO, Penta E, TH01, vWA, TPOX, and SE33), three Y-chromosomal loci (DYS391, DYS576, and DYS570), and Amelogenin. Study results demonstrate that the instrument is reliable, reproducible, accurate, robust, and ready for a large scale, comprehensive developmental validation by NDIS-participating laboratories. The additional loci in the FlexPlex assay allow for improved STR profile sharing globally, increase the power of discrimination for identification matches, and improve the effectiveness of kinship analyses.
快速 DNA 鉴定是利用坚固、可现场部署的系统在执法、军事、移民和国土安全应用中生成短串联重复序列 (STR) 图谱。使用 FlexPlex27 对 ANDE 快速 DNA 鉴定系统进行了性能验证研究，FlexPlex27 是一种高度多重化的 27 个基因座检测试剂盒，可生成 CODIS 核心基因座扩展和所有国际数据库所需的其他 STR 基因座的数据。该检测试剂盒包含 23 个常染色体基因座（D1S1656、D2S1338、D2S441、D3S1358、D5S81、D6S1043、D7S820、D8S1179、D10S1248、D12S391、D13S317、D16S539、D18S51、D19S433、D21S11、D22S1045、FGA、CSF1PO、Penta E、TH01、vWA、TPOX 和 SE33）、3 个 Y 染色体基因座（DYS391、DYS576 和 DYS570）和 Amelogenin。研究结果表明，该仪器可靠、可重复、准确、稳健，可由 NDIS 参与实验室进行大规模、全面的开发验证。FlexPlex 检测试剂盒中的额外基因座可实现全球范围内 STR 图谱共享的改善，提高识别匹配的区分能力，并提高亲属关系分析的效果。
