Mao Fei, Wu Yunbing, Tang Xudong, Wang Juanjuan, Pan Zhaoji, Zhang Peng, Zhang Bin, Yan Yongmin, Zhang Xu, Qian Hui, Xu Wenrong
Key Laboratory of Medical Science and Laboratory Medicine of Jiangsu Province, School of MedicineJiangsu University, 301 Xuefu Road, Zhenjiang, 212013, Jiangsu, People's Republic of China.
Jiangsu University of Science and Technology, Zhenjiang, 212018, Jiangsu, People's Republic of China.
Biotechnol Lett. 2017 Jun;39(6):929-938. doi: 10.1007/s10529-017-2315-4. Epub 2017 Mar 3.
To investigate the role of human umbilical cord mesenchymal stem cells (hucMSCs) in the treatment of dextran sulfate sodium (DSS)-induced inflammatory bowel disease (IBD).
ICG-hucMSCs homed to colon tissues of IBD mice 12 h after injection. The injection of hucMSCs significantly relieved the IBD symptoms and inflammatory cell infiltration. The expression of IL-10 gene increased while those of 15-LOX-1, TNF-α, IL-6, IL-1β, and IP-10 genes decreased in colon tissues and spleens of hucMSCs-treated mice. The activation of STAT3 was inhibited in colon tissues and spleens of IBD mice that were treated with hucMSCs. In addition, the percentage of macrophages decreased in colon tissues and spleens of hucMSCs-treated IBD mice. Moreover, we provided evidence that in vitro co-culture with hucMSCs inhibited the expression of 15-LOX-1, IL-6 and p-STAT3 in mouse enterocoelia macrophages.
HucMSCs alleviate DSS-induced IBD through the modulation of 15-LOX-1 in macrophages.
探讨人脐带间充质干细胞（hucMSCs）在治疗葡聚糖硫酸钠（DSS）诱导的炎症性肠病（IBD）中的作用。
注射后12小时，ICG-hucMSCs归巢至IBD小鼠的结肠组织。注射hucMSCs可显著缓解IBD症状和炎症细胞浸润。hucMSCs处理的小鼠结肠组织和脾脏中，IL-10基因表达增加，而15-LOX-1、TNF-α、IL-6、IL-1β和IP-10基因表达降低。hucMSCs处理的IBD小鼠结肠组织和脾脏中STAT3的激活受到抑制。此外，hucMSCs处理的IBD小鼠结肠组织和脾脏中巨噬细胞百分比降低。而且，我们提供的证据表明，与hucMSCs体外共培养可抑制小鼠腹腔巨噬细胞中15-LOX-1、IL-6和p-STAT3的表达。
hucMSCs通过调节巨噬细胞中的15-LOX-1来减轻DSS诱导的IBD。
