Lin Chin-Yin, Hsieh Yi-Hsien, Yang Shun-Fa, Chu Shu-Chen, Chen Pei-Ni, Hsieh Yih-Shou
Institute of Biochemistry, Microbiology and Immunology, Chung Shang Medical University, Taichung City, Taiwan.
Institute of Medicine, Chung Shan Medical University, Taichung, Taiwan.
Environ Toxicol. 2017 Jul;32(7):1878-1887. doi: 10.1002/tox.22410. Epub 2017 Mar 4.
Metastasis is the most common cause of cancer-related mortality in patients, and epithelial-mesenchymal transition (EMT) is essential for cancer metastasis and antidrug resistance. Cinnamomum cassia has several antioxidative, anti-inflammatory, and anticancer biological effects. However, the anti-EMT effect of C. cassia in human lung carcinoma is rarely reported. In this study, we determined whether C. cassia extracts (CCE) reduces the EMT and tumor growth of human lung adenocarcinoma cells. CCE inhibited the transforming growth factor (TGF)-β1-induced cell motility and invasiveness of A549 and H1299 cells by repressing matrix metalloproteinase-2 and urokinase-type plasminogen activator as well as impaired cell adhesion to collagen. CCE also affected the TGF-β1-induced EMT by downregulating the expression of vimentin and fibronectin and upregulating E-cadherin. The nude mice xenograft model showed that CCE reduced A549 tumor growth. Thus, CCE possesses antimetastatic activity of A549 and H1299 cells by affecting EMT and suppressing A549 tumor growth in vivo. This result suggested that CCE could be used as an antimetastatic agent or as an adjuvant for anticancer therapy.
转移是癌症患者与癌症相关死亡的最常见原因，上皮-间质转化（EMT）对于癌症转移和抗药性至关重要。肉桂具有多种抗氧化、抗炎和抗癌生物学效应。然而，肉桂对人肺癌的抗EMT作用鲜有报道。在本研究中，我们确定了肉桂提取物（CCE）是否能减少人肺腺癌细胞的EMT和肿瘤生长。CCE通过抑制基质金属蛋白酶-2和尿激酶型纤溶酶原激活剂以及损害细胞与胶原蛋白的黏附，抑制了转化生长因子（TGF）-β1诱导的A549和H1299细胞的迁移和侵袭。CCE还通过下调波形蛋白和纤连蛋白的表达以及上调E-钙黏蛋白来影响TGF-β1诱导的EMT。裸鼠异种移植模型显示CCE减少了A549肿瘤的生长。因此，CCE通过影响EMT和抑制体内A549肿瘤生长，具有A549和H1299细胞的抗转移活性。这一结果表明CCE可作为一种抗转移剂或抗癌治疗的佐剂。
