Yagnik Bhrugu, Sharma Drashya, Padh Harish, Desai Priti
Department of Cell and Molecular Biology, B. V. Patel Pharmaceutical Education and Research Development Centre, Ahmedabad, Gujarat.
B. R. D. School of Biosciences, Sardar Patel University, Vallabh Vidhyanagar, Gujarat.
Microbiol Immunol. 2017 Apr;61(3-4):123-129. doi: 10.1111/1348-0421.12473.
Food grade Lactococcus lactis has been widely used as an antigen and DNA delivery vehicle. We have previously reported the use of non-invasive L. lactis to deliver the newly constructed immunostimulatory DNA vaccine reporter plasmid, pPERDBY. In the present report, construction of dual recombinant L. lactis expressing internalin A of Listeria monocytogenes and harboring pPERDBY (LL InlA + pPERDBY) to enhance the efficiency of delivery of DNA by L. lactis is outlined. After confirmation and validation of LL InlA + pPERDBY, its DNA delivery potential was compared with previously developed non-invasive r- L. lactis::pPERDBY. The use of invasive L. lactis resulted in around threefold increases in the number of enhanced green fluorescent protein-expressing Caco-2 cells. These findings reinforce the prospective application of invasive strain of L. lactis for delivery of DNA/RNA and antigens.
食品级乳酸乳球菌已被广泛用作抗原和DNA递送载体。我们之前曾报道过使用非侵袭性乳酸乳球菌来递送新构建的免疫刺激DNA疫苗报告质粒pPERDBY。在本报告中，概述了构建表达单核细胞增生李斯特菌内化素A并携带pPERDBY的双重重组乳酸乳球菌（LL InlA + pPERDBY），以提高乳酸乳球菌递送DNA的效率。在确认和验证LL InlA + pPERDBY后，将其DNA递送潜力与先前开发的非侵袭性重组乳酸乳球菌::pPERDBY进行了比较。使用侵袭性乳酸乳球菌导致表达增强型绿色荧光蛋白的Caco-2细胞数量增加了约三倍。这些发现强化了侵袭性乳酸乳球菌菌株在递送DNA/RNA和抗原方面的潜在应用。
