Bio-Energy Research Center, Chonnam National University, Gwangju 61186, Republic of Korea.
Department of Bioenergy Science and Technology, Chonnam National University, Gwangju 61186, Republic of Korea.
Bioresour Technol. 2017 Jun;233:44-50. doi: 10.1016/j.biortech.2017.02.040. Epub 2017 Feb 12.
Microalgae biomass are useful resources in biofuel production. The objective of this study was to evaluate bioethanol production in response to Porphyridium cruemtum culture conditions. Enzymatic hydrolysis of seawater P. cruemtum (SPC) and freshwater P. cruemtum (FPC, 1% substrate loading, w/v) resulted in glucose conversion yields of 89.8 and 85.3%, respectively, without any pretreatment. However, FPC hydrolysate was more efficiently converted to ethanol about 7.1% than SPC hydrolysate. The comparison of separate hydrolysis and fermentation (SHF) and simultaneous saccharification and fermentation (SSF) showed that SSF processing is a superior method for bioethanol production from both SPC and FPC. Though SSF processing (5% substrate loading, w/v) in a 500-mL twin-neck round bottom flask, we achieved ethanol conversion yields of 65.4 and 70.3% from SPC and FPC, respectively, after 9h. These findings indicate that P. cruemtum can grow in freshwater conditions and is an efficient candidate for bioethanol production.
微藻生物质是生物燃料生产的有用资源。本研究旨在评估响应节旋藻培养条件的生物乙醇生产。未经任何预处理，海水节旋藻（SPC）和淡水节旋藻（FPC，1%底物负荷，w/v）的酶解分别产生 89.8%和 85.3%的葡萄糖转化率。然而，FPC 水解物比 SPC 水解物更有效地转化为乙醇，约为 7.1%。分批水解和发酵（SHF）与同步糖化和发酵（SSF）的比较表明，SSF 处理是从 SPC 和 FPC 生产生物乙醇的一种优越方法。尽管在 500 毫升双颈圆底烧瓶中进行 SSF 处理（5%底物负荷，w/v），但我们在 9 小时后分别从 SPC 和 FPC 获得了 65.4%和 70.3%的乙醇转化率。这些发现表明，节旋藻可以在淡水中生长，是生物乙醇生产的有效候选物。
