Centro de Biotecnologia Agrícola e Agro-Alimentar do Alentejo (CEBAL)/Instituto Politécnico de Beja (IPBeja), 7801-908 Beja, Portugal.
Centro de Biotecnologia Agrícola e Agro-Alimentar do Alentejo (CEBAL)/Instituto Politécnico de Beja (IPBeja), 7801-908 Beja, Portugal.
Bioresour Technol. 2015 Jun;186:309-315. doi: 10.1016/j.biortech.2015.03.037. Epub 2015 Mar 14.
The correct choice of the specific lignocellulosic biomass pretreatment allows obtaining high biomass conversions for biorefinery implementations and cellulosic bioethanol production from renewable resources. Cynara cardunculus (cardoon) pretreated by steam explosion (SE) was involved in second-generation bioethanol production using separate hydrolysis and fermentation (SHF) or simultaneous saccharification and fermentation (SSF) processes. Steam explosion pretreatment led to partial solubilisation of hemicelluloses and increased the accessibility of residual polysaccharides towards enzymatic hydrolysis revealing 64% of sugars yield against 11% from untreated plant material. Alkaline extraction after SE pretreatment of cardoon (CSEOH) promoted partial removal of degraded lignin, tannins, extractives and hemicelluloses thus allowing to double glucose concentration upon saccharification step. Bioethanol fermentation in SSF mode was faster than SHF process providing the best results: ethanol concentration 18.7 g L(-1), fermentation efficiency of 66.6% and a yield of 26.6g ethanol/100 g CSEOH or 10.1 g ethanol/100 g untreated cardoon.
正确选择特定的木质纤维素生物质预处理方法，可以实现高生物质转化率，从而从可再生资源中生产生物炼制和纤维素生物乙醇。采用蒸汽爆破（SE）预处理的蓟（cardoon）被用于使用分步水解和发酵（SHF）或同步糖化和发酵（SSF）工艺生产第二代生物乙醇。蒸汽爆破预处理导致半纤维素部分溶解，并增加了残余多糖对酶水解的可及性，显示出 64%的糖产率，而未经处理的植物材料仅为 11%。SE 预处理后用碱性提取蓟（CSEOH），可以促进部分降解木质素、单宁、提取物和半纤维素的去除，从而使糖化步骤中的葡萄糖浓度增加一倍。在 SSF 模式下进行生物乙醇发酵比 SHF 工艺更快，提供了最佳结果：乙醇浓度为 18.7 g L(-1)，发酵效率为 66.6%，CSEOH 或未处理的蓟 100 g 中分别有 26.6 g 乙醇和 10.1 g 乙醇。
