Yonemitsu N
Department of Pathology, Saga Medical School, Japan.
Cell Tissue Res. 1987 Dec;250(3):507-12. doi: 10.1007/BF00218941.
Outer and inner layer cells of bovine adrenal cortex were cultured separately to compare cellular structural characteristics and functional differences. Outer layer cells were polygonal in shape with radially distributed lipid droplets in the cytoplasm, and produced mainly aldosterone and cortisol. The aldosterone production increased upon stimulation with angiotensin II or dibutyryl-cAMP. In contrast, inner layer cells were spindle-shaped and had fine diffused lipid droplets. They produced four times as much cortisol as outer layer cells but no aldosterone. Cortisol production increased with ACTH or dibutyryl-cAMP stimulation. When stimulated by ACTH or by dibutyryl-cAMP, both types of adrenocortical cells showed "cellular retraction" whereby the number of cytoplasmic lipid droplets decreased and microvilli on the cellular surface increased. At the same time, the transverse distribution of actin fibers disappeared and the microtubules changed their distribution pattern from circular to radial. Stimulation by angiotensin II, on the other hand, brought no marked structural changes. These results indicate that, in functional terms, the outer layer cells and the inner layer cells in this culture system reflect zona glomerulosa and zona fasciculata-reticularis, respectively.
将牛肾上腺皮质的外层细胞和内层细胞分别进行培养，以比较细胞结构特征和功能差异。外层细胞呈多边形，细胞质中有呈放射状分布的脂滴，主要产生醛固酮和皮质醇。用血管紧张素II或二丁酰环磷腺苷刺激后，醛固酮分泌增加。相比之下，内层细胞呈纺锤形，有细小的弥散性脂滴。它们产生的皮质醇是外层细胞的四倍，但不产生醛固酮。促肾上腺皮质激素或二丁酰环磷腺苷刺激后，皮质醇分泌增加。当受到促肾上腺皮质激素或二丁酰环磷腺苷刺激时，两种肾上腺皮质细胞均出现“细胞回缩”，即细胞质脂滴数量减少，细胞表面微绒毛增加。同时，肌动蛋白纤维的横向分布消失，微管的分布模式从环状变为放射状。另一方面，血管紧张素II刺激未引起明显的结构变化。这些结果表明，从功能角度来看，该培养系统中的外层细胞和内层细胞分别反映了球状带和束状带-网状带。
