Yonemitsu N, Toda S, Takatori O, Miyabara S, Sugihara H
Department of Pathology, Saga Medical School, Japan.
Acta Pathol Jpn. 1991 Jun;41(6):428-36. doi: 10.1111/j.1440-1827.1991.tb03209.x.
Subcapsular small cortical cells (SC cells) and subcapsular large cortical cells (LC cells) of bovine adrenal cortex were cultured separately after purification by unit gravity sedimentation, and then compared with inner-half layer cells (IL cells) prepared by the same method. Both SC and LC cells were polygonal in shape and their mitochondria were elongated with lamellar cristae. SC cells became as large as LC cells on day 6 of culture with increased cytoplasmic lipid droplets, whereas IL cells showed no change in size. IL cells were spindle-shaped and had mitochondria with tubulovesicular cristae. Both SC and LC cells produced 11 beta-deoxycorticosterone, corticosterone, aldosterone and small amounts of 17 alpha-hydroxyprogesterone (17 alpha-OH-Prog) and cortisol (F). IL cells produced much more 17 alpha-OH-Prog and F than SC or LC cells. When stimulated with ACTH, cortical cells in each group showed cellular retraction and their mitochondria became spherical. The amounts of 17 alpha-OH-Prog and F increased in all groups, especially in IL cells. These results show that LC cells have similar characteristics to SC cells in both morphology and function, and that they differ from IL cells, which correspond to classical fasciculata-reticularis cells.
牛肾上腺皮质的被膜下小皮质细胞（SC细胞）和被膜下大皮质细胞（LC细胞）经单位重力沉降纯化后分别培养，然后与用相同方法制备的内半层细胞（IL细胞）进行比较。SC细胞和LC细胞均呈多边形，其线粒体呈细长形，有板层嵴。培养第6天时，SC细胞变得与LC细胞一样大，胞质脂滴增多，而IL细胞大小无变化。IL细胞呈纺锤形，线粒体有管状泡状嵴。SC细胞和LC细胞均产生11β-脱氧皮质酮、皮质酮、醛固酮以及少量的17α-羟孕酮（17α-OH-Prog）和皮质醇（F）。IL细胞产生的17α-OH-Prog和F比SC细胞或LC细胞多得多。用促肾上腺皮质激素（ACTH）刺激时，每组皮质细胞均出现细胞收缩，其线粒体变为球形。所有组中17α-OH-Prog和F的量均增加，尤其是IL细胞。这些结果表明，LC细胞在形态和功能上与SC细胞具有相似的特征，并且它们与对应于经典束状带-网状带细胞的IL细胞不同。
