Liu M Y, Zhang L J
Department of Cardiology, Beijing Anzhen Hospital, Capital Medical University, Beijing 100029, China.
Zhonghua Xin Xue Guan Bing Za Zhi. 2017 Feb 24;45(2):137-141. doi: 10.3760/cma.j.issn.0253-3758.2017.02.013.
To explore the effect of trimetazidine pre-treatments on serum 5-hydroxytryptamine (5-HT) and serotonin transporter (SERT), platelet 5-HT and SERT in the Sprague Dawley rats with myocardial infarction(MI), depression, and myocardial infarction co-exist with depression (MI+ depression) and in sham operated rats. Eighty rats were divided into treatment group and placebo (saline) group by the random number table method (=40 each group). After 4 weeks' treatment with trimetazidine, or saline, these rats were assigned to respective sham subgroup, depression subgroup, MI subgroup, MI + depression subgroup (=10 each subgroup). All rats were sacrificed 3 days later. 5-HT in serum and platelet, and SERT in serum and platelet of the rats were tested by enzyme linked immunosorbent assay (ELISA). Data were analyzed by SPSS 17.0. In saline group, serum and platelet 5-HT levels were significantly lower in MI, depression and MI+ depression subgroup compared to sham subgroup (all <0.01). In trimetazidine pre-treatment group, serum and platelet 5-HT levels were significantly higher in the depression subgroup, MI subgroup, MI + depression subgroup than respective subgroups of saline pretreated rats (all <0.01). In saline group, SERT in serum and platelet were higher in MI, depression, MI+ depression subgroup than the sham subgroup and statistical significance was found between MI+ depression subgroup and sham subgroups. Serum SERT level was significantly lower in the treatment group compared to saline group for sham rats (<0.01). In treatment group, serum SERT level was higher in MI, depression and MI+ depression subgroups compared to respective subgroup treated with saline while the statistical significance was found in depression subgroup between trimetazidine pre-treatment and saline control groups. Platelet SERT was higher in MI, depression and MI+ depression subgroups compared to sham subgroup post saline treatment. Platelet SERT was significantly lower in MI, depression and MI+ depression subgroups post trimetazidine pre-treatment than in respective subgroups post saline treatment (all <0.01). Our study results indicate that trimetazidine pretreatment could increase the levels of 5-HT and SERT in serum and 5-HT in platelet, and decrease the level of SERT in platelet in the rat MI and depression model.
探讨曲美他嗪预处理对心肌梗死（MI）、抑郁以及心肌梗死合并抑郁（MI+抑郁）的Sprague Dawley大鼠和假手术大鼠血清5-羟色胺（5-HT）、5-羟色胺转运体（SERT）、血小板5-HT和SERT的影响。80只大鼠采用随机数字表法分为治疗组和安慰剂（生理盐水）组（每组=40只）。用曲美他嗪或生理盐水治疗4周后，将这些大鼠分为各自的假手术亚组、抑郁亚组、MI亚组、MI+抑郁亚组（每个亚组=10只）。3天后处死所有大鼠。采用酶联免疫吸附测定（ELISA）法检测大鼠血清和血小板中的5-HT以及血清和血小板中的SERT。数据用SPSS 17.0软件进行分析。在生理盐水组中，与假手术亚组相比，MI、抑郁和MI+抑郁亚组的血清和血小板5-HT水平显著降低（均<0.01）。在曲美他嗪预处理组中，抑郁亚组、MI亚组、MI+抑郁亚组的血清和血小板5-HT水平显著高于生理盐水预处理大鼠的相应亚组（均<0.01）。在生理盐水组中，MI、抑郁、MI+抑郁亚组血清和血小板中的SERT高于假手术亚组，且MI+抑郁亚组与假手术亚组之间存在统计学差异。与生理盐水组相比，治疗组假手术大鼠的血清SERT水平显著降低（<0.01）。在治疗组中，与生理盐水治疗的相应亚组相比，MI、抑郁和MI+抑郁亚组的血清SERT水平较高，而曲美他嗪预处理组和生理盐水对照组在抑郁亚组中存在统计学差异。生理盐水治疗后，MI、抑郁和MI+抑郁亚组的血小板SERT高于假手术亚组。曲美他嗪预处理后，MI亚组、抑郁亚组和MI+抑郁亚组的血小板SERT显著低于生理盐水治疗后的相应亚组（均<0.01）。我们的研究结果表明，曲美他嗪预处理可提高大鼠MI和抑郁模型血清中5-HT和SERT水平以及血小板中5-HT水平，并降低血小板中SERT水平。
