Department of Cardiology, Beijing Anzhen Hospital, Capital Medical University, Beijing 100029, China.
Department of Neurosciences, Allegheny College, Meadville 16335, USA.
Chin Med J (Engl). 2017 Sep 20;130(18):2219-2225. doi: 10.4103/0366-6999.213966.
: Psychocardiological researches have suggested a central role of 5-hydroxytryptamine (5-HT) on psychocardiological mechanism. This study aimed to further explore the central role of 5-HT and pretreatment effects of XinLingWan on rats with myocardial infarction (MI) and/or depression.
: Ninety Sprague-Dawley rats were randomly divided into three groups: MI group, depression group, and MI + depression group (n = 30 in each group). Each group was then divided into three subgroups (n = 10 in each subgroup): a negative control subgroup (NCS), a Western medicine subgroup (WMS), and a traditional Chinese medicine subgroup (TCMS), which were received pretreatment once a day for 4 weeks by saline, 20 mg/kg sertraline mixed with 2 ml saline, and 40 mg/kg XingLingWan mixed with 2 ml saline, respectively. Different rat models were established after different pretreatments. Rats were then sacrificed for detection of serum 5-HT, platelet 5-HT, 5-HT receptors (5-HTR), and serotonin transporter (SERT). Data were analyzed by one-way analysis of variance (ANOVA) and least-significant difference (LSD) testing.
: MI group: compared with NCS, there was a significant increase in WMS and TCMS of serum 5-HT (176.15 ± 11.32 pg/ml vs. 334.50 ± 29.09 pg/ml and 474.04 ± 10.86 pg/ml, respectively, both P = 0.000), platelet 5-HT (129.74 ± 27.17 pg/ml vs. 322.24 ± 11.60 pg/ml and 340.4 5 ± 17.99 pg/ml, respectively, both P = 0.000); depression group: compared with NCS, there was a significant increase in WMS and TCMS of serum 5-HT (194.69 ± 5.09 pg/ml vs. 326.21 ± 39.98 pg/ml and 456.33 ± 23.12 pg/ml, respectively, both P = 0.000), platelet 5-HT (175.15 ± 4.07 pg/ml vs. 204.56 ± 18.59 pg/ml and 252.03 ± 22.26 pg/ml, respectively, P = 0.004 and P = 0.000, respectively); MI + depression group: compared with NCS, there was a significant increase in both WMS and TCMS of serum 5-HT (182.50 ± 10.23 pg/ml vs. 372.55 ± 52.23 pg/ml and 441.76 ± 23.38 pg/ml, respectively, both P = 0.000) and platelet 5-HT (180.83 ± 11.08 pg/ml vs. 221.12 ± 22.23 pg/ml and 265.37 ± 29.49 pg/ml, respectively, P = 0.011 and P = 0.000, respectively).
: By elevating the amount of 5-HT and modulating 5-HTR and SERT levels in serum and platelets, XinLingWan and sertraline were found to exert pretreatment effect on rat models of MI and/or depression.
心理心脏病学研究表明，5-羟色胺（5-HT）在心理心脏病学机制中起核心作用。本研究旨在进一步探讨 5-HT 的核心作用，以及心宁丸预处理对心肌梗死（MI）和/或抑郁症大鼠的影响。
90 只 Sprague-Dawley 大鼠随机分为三组：MI 组、抑郁组和 MI+抑郁组（每组 30 只）。每组又分为三个亚组（每组 10 只）：阴性对照组（NCS）、西药组（WMS）和中药组（TCMS），分别给予生理盐水、20mg/kg 舍曲林混合 2ml 生理盐水和 40mg/kg 心宁丸混合 2ml 生理盐水每日预处理 1 次，共 4 周。在不同的预处理后建立不同的大鼠模型。然后处死大鼠，检测血清 5-HT、血小板 5-HT、5-HT 受体（5-HTR）和血清素转运蛋白（SERT）。采用单因素方差分析（ANOVA）和最小显著差异（LSD）检验进行数据分析。
MI 组：与 NCS 相比，WMS 和 TCMS 的血清 5-HT 水平显著升高（176.15±11.32pg/ml 比 334.50±29.09pg/ml 和 474.04±10.86pg/ml，均 P=0.000），血小板 5-HT 水平也显著升高（129.74±27.17pg/ml 比 322.24±11.60pg/ml 和 340.45±17.99pg/ml，均 P=0.000）；抑郁组：与 NCS 相比，WMS 和 TCMS 的血清 5-HT 水平显著升高（194.69±5.09pg/ml 比 326.21±39.98pg/ml 和 456.33±23.12pg/ml，均 P=0.000），血小板 5-HT 水平也显著升高（175.15±4.07pg/ml 比 204.56±18.59pg/ml 和 252.03±22.26pg/ml，P=0.004 和 P=0.000）；MI+抑郁组：与 NCS 相比，WMS 和 TCMS 的血清 5-HT 水平均显著升高（182.50±10.23pg/ml 比 372.55±52.23pg/ml 和 441.76±23.38pg/ml，均 P=0.000），血小板 5-HT 水平也显著升高（180.83±11.08pg/ml 比 221.12±22.23pg/ml 和 265.37±29.49pg/ml，P=0.011 和 P=0.000）。
通过升高 5-HT 的含量，调节血清和血小板中 5-HTR 和 SERT 的水平，心宁丸和舍曲林对 MI 和/或抑郁症大鼠模型具有预处理作用。
