Nogueira Lílian, Mello Danielle Ferraz, Trevisan Rafael, Garcia Danielly, da Silva Acosta Daiane, Dafre Alcir Luiz, de Almeida Eduardo Alves
Laboratório de Biomarcadores de Contaminação Aquática, Departamento de Química e Ciências Ambientais, Universidade Estadual Paulista (IBILCE/UNESP), 15054-000, São José do Rio Preto, SP, Brazil.
Laboratório de Defesas Celulares, Departamento de Bioquímica, Universidade Federal de Santa Catarina, 88040-900, Florianópolis, SC, Brazil.
Mar Environ Res. 2017 May;126:109-115. doi: 10.1016/j.marenvres.2017.02.009. Epub 2017 Feb 24.
This study investigated the effects of hypoxia on oxidative stress response and immune function in mussels Perna perna exposed to air for 6, 12, 24 and 48 h. In air-exposed mussels, the antioxidant enzymes superoxide dismutase (SOD), catalase, and glutathione reductase (GR) activities were lower in gill tissues (24-48 h) and digestive gland (12 h), while the glutathione peroxidase and GR activities were increased in the digestive gland (48 h). In both tissues, aerial exposure promoted a rapid (6 h) and persistent (up to 48 h) increase of glutathione levels. Decreased hemocyte count and viability, as well as increased phagocytic activity and cellular adhesion capacity were detected after prolonged aerial exposure (>12 h). In summary, induction of thiol pools, altered antioxidant enzyme activities, and activation of immune responses were detected in hypoxia exposed brown mussels, indicating hypoxia induced tissue-specific responses in both antioxidant and immune systems.
本研究调查了缺氧对暴露于空气中6、12、24和48小时的翡翠贻贝氧化应激反应和免疫功能的影响。在暴露于空气的贻贝中，鳃组织（24 - 48小时）和消化腺（12小时）中的抗氧化酶超氧化物歧化酶（SOD）、过氧化氢酶和谷胱甘肽还原酶（GR）活性降低，而消化腺（48小时）中的谷胱甘肽过氧化物酶和GR活性增加。在这两种组织中，暴露于空气中均促进了谷胱甘肽水平快速（6小时）且持续（长达48小时）的升高。长时间暴露于空气中（>12小时）后，检测到血细胞计数和活力降低，以及吞噬活性和细胞黏附能力增加。总之，在缺氧暴露的褐贻贝中检测到硫醇池的诱导、抗氧化酶活性的改变以及免疫反应的激活，表明缺氧在抗氧化和免疫系统中均诱导了组织特异性反应。
