Lei Xiangyu, Yang Lishi, Tan Liqi, Yang Qibin, Zhou Falin, Jiang Shigui, Huang Jianhua
Shenzhen Base of South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shenzhen 518108, China.
College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China.
Animals (Basel). 2023 Jan 28;13(3):462. doi: 10.3390/ani13030462.
Air exposure is an important environmental stressor during the transportation and cultivation of . We evaluated the effect of re-submersion for 24 h after dry transportation for 24 h on the histological structure, antioxidant activity, and gene expression of crayfish. The antioxidant parameters of catalase (CAT), superoxide dismutase (SOD), malondialdehyde (MDA), and lactate dehydrogenase (LDH), and the relative expression of CAT, SOD, HSP70, and ferritin genes were subsequently measured in the hepatopancreas and gills at both stages. Histopathology found that air exposure led to vacuolation of the hepatopancreas and disorderly arrangement of respiratory epithelial cells (REC) in the gills. The activities of catalase (CAT), superoxide dismutase (SOD), malondialdehyde (MDA), and lactic dehydrogenase (LDH) in the hepatopancreas and gills increased with short-term air exposure. The relative expression of genes (CAT, SOD, HSP70, and Ferritin) were induced after short-term air exposure. During re-submersion, MDA content and CAT and SOD activities in the hepatopancreas and gills were restored after 24 h, however, LDH activity and hepatopancreatic tissue damage were not repaired. Our results indicate that air exposure can cause oxidative damage to , and CAT and SOD can be used to determine the response of crayfish exposed to air, in addition to some damage that can be eliminated after re-submersion to a limited degree. This study provides foundational data that re-submersion can improve crayfish performance under hypoxic stress to a certain extent and will lead to the development of more effective transportation strategies and decrease economic losses in the future.
在[具体生物]的运输和养殖过程中，空气暴露是一个重要的环境应激源。我们评估了在干运24小时后再浸没24小时对小龙虾组织学结构、抗氧化活性和基因表达的影响。随后在两个阶段测量了肝胰腺和鳃中过氧化氢酶（CAT）、超氧化物歧化酶（SOD）、丙二醛（MDA）和乳酸脱氢酶（LDH）的抗氧化参数，以及CAT、SOD、热休克蛋白70（HSP70）和铁蛋白基因的相对表达。组织病理学发现，空气暴露导致肝胰腺空泡化和鳃中呼吸上皮细胞（REC）排列紊乱。短期空气暴露后，肝胰腺和鳃中过氧化氢酶（CAT）、超氧化物歧化酶（SOD）、丙二醛（MDA）和乳酸脱氢酶（LDH）的活性增加。短期空气暴露后，基因（CAT、SOD、HSP70和铁蛋白）的相对表达被诱导。在再浸没过程中，肝胰腺和鳃中的MDA含量以及CAT和SOD活性在24小时后恢复，然而，LDH活性和肝胰腺组织损伤并未修复。我们的结果表明，空气暴露会对[具体生物]造成氧化损伤，CAT和SOD可用于确定小龙虾对空气暴露的反应，此外，一些损伤在再浸没后可在一定程度上消除。本研究提供了基础数据，表明再浸没可以在一定程度上提高小龙虾在缺氧应激下的性能，并将导致未来开发更有效的运输策略，减少经济损失。
