Martínez-Paz Pedro, Morales Mónica, Urien Josune, Morcillo Gloria, Martínez-Guitarte José Luis
Grupo de Biología y Toxicología Ambiental. Departamento de Física Matemática y de Fluidos, Facultad de Ciencias, Universidad Nacional de Educación a Distancia (UNED), C/ Senda del Rey 9, 28040 Madrid, Spain.
Grupo de Biología y Toxicología Ambiental. Departamento de Física Matemática y de Fluidos, Facultad de Ciencias, Universidad Nacional de Educación a Distancia (UNED), C/ Senda del Rey 9, 28040 Madrid, Spain.
Ecotoxicol Environ Saf. 2017 Jun;140:185-190. doi: 10.1016/j.ecoenv.2017.02.047. Epub 2017 Mar 8.
Triclosan (TCS) is an antibacterial agent widely used in personal care and consumer products and commonly detected in aquatic ecosystems. In the present study, the effects of TCS on endocrine-related genes of Chironomus riparius aquatic larvae, a reference organism in aquatic toxicology, were evaluated. Twenty-four-hour in vivo exposures at 10µg/L, 100µg/L, and 1000µg/L TCS revealed that this xenobiotic was able to alter the transcriptional activity of ecdysone receptor gene (EcR), the ultraspiracle gene (usp), the estrogen-related receptor gene (ERR), and the E74 early ecdysone-inducible gene, as measured by real-time RT-PCR. Moreover, the hsp70 gene, a heat shock protein gene, was upregulated after exposure to TCS. The results of the present work provide the first evidence of the potential disruptive effects of TCS in endocrine-related genes suggesting a mode of action that mimics ecdysteroid hormones in insects.
三氯生（TCS）是一种广泛用于个人护理和消费品的抗菌剂，在水生生态系统中普遍存在。在本研究中，评估了TCS对水生毒理学中的参考生物——摇蚊水生幼虫内分泌相关基因的影响。在10μg/L、100μg/L和1000μg/L的TCS浓度下进行24小时体内暴露实验，结果显示，通过实时逆转录聚合酶链反应（RT-PCR）检测发现，这种外源性物质能够改变蜕皮激素受体基因（EcR）、超气门蛋白基因（usp）、雌激素相关受体基因（ERR）以及E74早期蜕皮激素诱导基因的转录活性。此外，热休克蛋白基因hsp70在暴露于TCS后表达上调。本研究结果首次证明了TCS对内分泌相关基因具有潜在的破坏作用，提示其作用模式类似于昆虫中的蜕皮甾体激素。
