Martins Luís Miguel Lourenço, Marques Andreia Grilo, Pereira Luísa Maria Dotti Silva, Semião-Santos Saul José, Bento Ofélia Pereira
Department of Veterinary Medicine, School of Sciences and Technology, University of Évora, Évora, Portugal; Institute of Mediterranean Agricultural and Environmental Science (ICAAM), University of Évora, Évora, Portugal.
Department of Veterinary Medicine, School of Sciences and Technology, University of Évora, Évora, Portugal.
Postepy Dermatol Alergol. 2017 Feb;34(1):60-69. doi: 10.5114/ada.2017.65623. Epub 2017 Feb 7.
Much less is known about grass-pollen allergens to dogs, when compared with humans. Genetic-based patterns might play an important role in sensitization profiles, conditioning the success of allergen-specific immunotherapy.
Mapping of () and () allergens for grass pollen-sensitized atopic dogs, for better understanding how individual allergograms may influence the response to grass-pollen immunotherapy.
To identify and allergoms for dogs, 15 individuals allergic to grass pollen and sensitized to were selected. and proteomes were separated by isoelectric focusing (IEF), one-dimensional (1-D) and two-dimensional (2-D) sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). Separated proteins were blotted onto (PVDF) membranes and allergens were identified by patient sera IgE in Western Blotting (WB).
In , 17 allergens were identified from IEF and 11 from 1-D SDS-PAGE, while from , 18 and 6 allergens were identified, respectively. From 2-D SDS-PAGE 13 spots were identified from and 27 from .
Several similarities were found between dog and human and sensitization profiles but no relationship between clinical signs and a specific pattern of allergen recognition was observed. Similarities were found in each patient pattern of sensitization between and , also suggesting cross-reactive phenomena. Further molecular epidemiology approach is needed to understand the role of the sensitization pattern in allergen-specific immunotherapy effectiveness in grass-pollen allergic dogs.
与人类相比，我们对犬类草花粉过敏原的了解要少得多。基于基因的模式可能在致敏谱中发挥重要作用，影响过敏原特异性免疫疗法的成功率。
绘制对草花粉致敏的特应性犬的（）和（）过敏原图谱，以更好地了解个体过敏谱如何影响对草花粉免疫疗法的反应。
为了鉴定犬类的和过敏原，选择了15只对草花粉过敏且对致敏的个体。和蛋白质组通过等电聚焦（IEF）、一维（1-D）和二维（2-D）十二烷基硫酸钠聚丙烯酰胺凝胶电泳（SDS-PAGE）进行分离。分离后的蛋白质印迹到（PVDF）膜上，通过患者血清IgE在蛋白质印迹法（WB）中鉴定过敏原。
在中，从IEF鉴定出17种过敏原，从1-D SDS-PAGE鉴定出11种，而从中分别鉴定出18种和6种过敏原。从2-D SDS-PAGE中，从鉴定出13个斑点，从鉴定出27个斑点。
在犬类和人类的和致敏谱之间发现了一些相似之处，但未观察到临床症状与特定过敏原识别模式之间的关系。在每个患者的和致敏模式之间也发现了相似之处，这也表明存在交叉反应现象。需要进一步的分子流行病学方法来了解致敏模式在草花粉过敏犬过敏原特异性免疫疗法有效性中的作用。
