Kubota Shota, Miyaji Kazuki, Shimo Yusaku, Shimakura Hidekatsu, Takase Yukari, Okamoto Noriaki, Kiuchi Akio, Fujimura Masato, Fujimura Takashi, DeBoer Douglas J, Tsukui Toshihiro, Sakaguchi Masahiro
Department of Veterinary Microbiology, School of Veterinary Medicine, Azabu University, 1-17-71 Fuchinobe, Chuo-ku, Sagamihara, Kanagawa 252-5201, Japan.
Vet Immunol Immunopathol. 2012 Sep 15;149(1-2):132-5. doi: 10.1016/j.vetimm.2012.06.007. Epub 2012 Jun 15.
The present study investigated IgE reactivity to a new Cryptomeria japonica pollen allergen (Cry j 3) in dogs with atopic dermatitis by using a fluorometric ELISA. Serum samples from 15 dogs that showed IgE sensitivity to crude C. japonica pollen allergen by ELISA were tested for specific IgE to each allergen, individually. All 15 dogs had anti-Cry j 1 IgE, 6 (40%) had anti-Cry j 2 IgE, and 11 (73%) had anti-Cry j 3 IgE. Further, we found that these anti-Cry j 3 IgE reacted to Cry j 3 with immunoblotting analysis. These findings indicate that Cry j 3 may be a major allergen in dogs.
本研究通过荧光酶联免疫吸附测定法，调查了患有特应性皮炎的犬对一种新的日本柳杉花粉过敏原（Cry j 3）的IgE反应性。对15只通过酶联免疫吸附测定法显示对粗制日本柳杉花粉过敏原具有IgE敏感性的犬的血清样本，分别检测其对每种过敏原的特异性IgE。所有15只犬均具有抗Cry j 1 IgE，6只（40%）具有抗Cry j 2 IgE，11只（73%）具有抗Cry j 3 IgE。此外，我们通过免疫印迹分析发现，这些抗Cry j 3 IgE与Cry j 3发生反应。这些发现表明，Cry j 3可能是犬的主要过敏原。
