Dieter P, Schulze-Specking A, Karck U, Decker K
Biochemisches Institut der Universität Freiburg im Breisgau, Federal Republic of Germany.
Eur J Biochem. 1987 Dec 30;170(1-2):201-6. doi: 10.1111/j.1432-1033.1987.tb13687.x.
The release of the prostaglandins E2 and D2, induced by zymosan and phorbol ester in cultured rat Kupffer cells, was found to depend on the extracellular concentration of Na+. Eicosanoid formation following the administration of the Ca2+ ionophore A23187 or of arachidonic acid, however, did not require the presence of sodium ions in the medium. A half-maximal rate of prostaglandin release by zymosan-treated Kupffer cells was obtained between 4 mM and 5 mM Na+; and a Na+ concentration of greater than or equal to 30 mM was required to maximally stimulate prostaglandin E2 and D2 formation in the cultured liver macrophages. In contrast, the superoxide production following the administration of zymosan or of phorbol ester was quite independent of extracellular Na+. The zymosan and phorbol-ester-stimulated release of prostaglandins E2 and D2 was inhibited by amiloride. Artificial intracellular alkalization enhanced the prostanoid production of unstimulated and of zymosan-stimulated cells whereas artificial intracellular acidification inhibited the zymosan-elicited prostaglandin synthesis. In contrast, the superoxide formation was independent of the pH changes. The data presented here suggest that the prostaglandin production elicited by zymosan or phorbol ester in cultured rat Kupffer cells requires an activated Na+/H+ exchange.
研究发现，酵母聚糖和佛波酯在培养的大鼠库普弗细胞中诱导产生的前列腺素E2和D2的释放，取决于细胞外Na+的浓度。然而，在给予Ca2+离子载体A23187或花生四烯酸后，类花生酸的形成并不需要培养基中存在钠离子。酵母聚糖处理的库普弗细胞释放前列腺素的半最大速率在4 mM至5 mM Na+之间获得；培养的肝巨噬细胞中，需要大于或等于30 mM的Na+浓度才能最大程度地刺激前列腺素E2和D2的形成。相比之下，给予酵母聚糖或佛波酯后产生的超氧化物与细胞外Na+无关。阿米洛利可抑制酵母聚糖和佛波酯刺激的前列腺素E2和D2的释放。人工细胞内碱化可增强未刺激细胞和酵母聚糖刺激细胞的前列腺素生成，而人工细胞内酸化则抑制酵母聚糖引发的前列腺素合成。相比之下，超氧化物的形成与pH变化无关。此处呈现的数据表明，酵母聚糖或佛波酯在培养的大鼠库普弗细胞中引发的前列腺素生成需要激活的Na+/H+交换。
