INRS-Institut Armand-Frappier, Edifice 18, 531 blvd. des Prairies, Laval (QC) H7V 1B7, Canada.
INRS-Institut Armand-Frappier, Edifice 18, 531 blvd. des Prairies, Laval (QC) H7V 1B7, Canada.
Mar Pollut Bull. 2017 May 15;118(1-2):237-247. doi: 10.1016/j.marpolbul.2017.02.041. Epub 2017 Mar 2.
The potential risk of pharmaceuticals in the environment to top-predators is still largely unknown. In this study, we assessed the immunotoxic effects of ten pharmaceuticals individually and as mixtures on a harbor seal (Phoca vitulina) B lymphoma cell line. A significant reduction in lymphocyte transformation was observed following an exposure to 12,500μg/L 17α-ethinyl estradiol and 25,000μg/L naproxen. Exposure to 12,500μg/L 17α-ethinyl estradiol decreased the percentage of cell in the G/G phase of the cell cycle while increasing the percentage of cells in the S phase. Carbamazepine exposure increased the amount of cells in the G/M phase. Binary mixtures showed synergistic effects in lymphocyte transformation, cell cycle and apoptosis assays. Concentrations inducing toxic effects in the cell line were similar to those affecting fish in previous studies. A reduction of functional activities of the immune system may lead to altered host resistance to pathogens in free-ranging pinnipeds.
环境中的药物对顶级捕食者的潜在风险在很大程度上仍然未知。在这项研究中，我们评估了十种药物单独和混合物对港湾海豹（Phoca vitulina）B 淋巴瘤细胞系的免疫毒性作用。暴露于 17α-乙炔基雌二醇 12500μg/L 和萘普生 25000μg/L 后，淋巴细胞转化明显减少。暴露于 17α-乙炔基雌二醇 12500μg/L 会降低细胞周期 G/G 期的细胞百分比，同时增加 S 期的细胞百分比。卡马西平暴露会增加 G/M 期的细胞数量。二元混合物在淋巴细胞转化、细胞周期和细胞凋亡试验中表现出协同作用。在细胞系中诱导毒性作用的浓度与先前研究中影响鱼类的浓度相似。免疫系统功能活动的减少可能导致自由放养鳍足类动物对病原体的宿主抵抗力发生改变。
