INRS-Institut Armand-Frappier, Edifice 18, 531 Blvd. des Prairies, Laval, QC H7V 1B7, Canada.
INRS-Institut Armand-Frappier, Edifice 18, 531 Blvd. des Prairies, Laval, QC H7V 1B7, Canada.
Mar Pollut Bull. 2018 Feb;127:225-234. doi: 10.1016/j.marpolbul.2017.12.001. Epub 2017 Dec 8.
The ubiquity of pharmaceuticals in the aquatic environment and the accumulation in organisms of lower trophic levels have been documented. The immunotoxicity of these xenobiotics has however been little investigated. This study assessed the effects of pharmaceuticals on the immune responses of harbor seal lymphocytes. Peripheral blood mononuclear cells isolated from harbor seal pups were exposed to varying concentrations of 17α-ethinyl estradiol (250-50,000μg/L), naproxen (500-100,000μg/L), carbamazepine (500-100,000μg/L), erythromycin (750-150,000μg/L) and binary mixtures thereof in vitro. All individual compounds and mixtures inhibited lymphocyte proliferation. Mixture effects were non-additive and predictive values overestimated the inhibition of proliferation. Male pups were more sensitive to erythromycin exposure. Comparison with the sensitivity of the 11B7501 cell line showed a higher sensitivity of pups to individual compounds and the inverse trend for mixtures. Based on our results, we hypothesize that pharmaceuticals may have the potential to interrupt immune functions in harbor seals.
已记录到药品在水生环境中的普遍性和在较低营养级生物体内的积累。然而，这些外来生物的免疫毒性却很少被研究。本研究评估了药物对港湾海豹淋巴细胞免疫反应的影响。从港湾海豹幼崽中分离出外周血单核细胞，将其暴露于不同浓度的 17α-乙炔雌二醇（250-50000μg/L）、萘普生（500-100000μg/L）、卡马西平（500-100000μg/L）、红霉素（750-150000μg/L）及其混合物中进行体外实验。所有单一化合物和混合物均抑制淋巴细胞增殖。混合物的作用是非加性的，预测值高估了增殖抑制作用。雄性幼崽对红霉素暴露更为敏感。与 11B7501 细胞系的敏感性进行比较表明，幼崽对单一化合物的敏感性更高，而混合物则相反。基于我们的结果，我们假设药物可能有潜力干扰港湾海豹的免疫功能。
