Iwaoka T, Tabata F, Takahashi T
Analytical and Metabolic Research Laboratories, Sankyo Co., Ltd., Tokyo, Japan.
Free Radic Biol Med. 1987;3(5):329-33. doi: 10.1016/s0891-5849(87)80041-2.
This article emphasizes the advantages of using a luminescence spectrometer based on photon counting techniques for the detection of lipid peroxidation. An overview is presented of how chemiluminescence can be stimulated in the luminol-cytochrome c heme peptide system as an assay for lipid hydroperoxides. This method is used for finding antioxidant drugs. The specificity and advantages of the chemiluminescent method for detecting lipid hydroperoxides is reviewed.
本文强调了使用基于光子计数技术的发光光谱仪检测脂质过氧化的优势。概述了如何在鲁米诺-细胞色素c血红素肽系统中激发化学发光,以此作为脂质氢过氧化物的一种检测方法。该方法用于寻找抗氧化药物。本文还综述了检测脂质氢过氧化物的化学发光方法的特异性和优势。