通过高效液相色谱法和异鲁米诺化学发光分析法检测皮摩尔水平的脂质氢过氧化物和过氧化氢。
Detection of lipid hydroperoxides and hydrogen peroxide at picomole levels by an HPLC and isoluminol chemiluminescence assay.
作者信息
Yamamoto Y, Ames B N
机构信息
Department of Biochemistry, University of California, Berkeley 94720.
出版信息
Free Radic Biol Med. 1987;3(5):359-61. doi: 10.1016/s0891-5849(87)80048-5.
PMID:3319802
Abstract
An isoluminol assay is utilized for the detection of hydrogen peroxide and lipid hydroperoxides in biological samples. The combination of this assay as a post-column detection for HPLC avoids interference of antioxidants and enables characterization of hydroperoxides at picomole levels. Two useful HPLC conditions for the separation of hydrogen peroxide, lipid hydroperoxides, antioxidants, and unoxidized lipids are described.
摘要
采用异鲁米诺分析法检测生物样品中的过氧化氢和脂质氢过氧化物。该分析法作为高效液相色谱（HPLC）的柱后检测方法，可避免抗氧化剂的干扰，并能对皮摩尔水平的氢过氧化物进行表征。文中描述了两种用于分离过氧化氢、脂质氢过氧化物、抗氧化剂和未氧化脂质的有效HPLC条件。
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