Zhang Aozhe, Chang Dingran, Zhang Zijian, Li Fan, Li Weihong, Wang Xu, Li Yingfu, Hua Qian
School of Basic Medical Science, Beijing University of Chinese Medicine, 11 East Road, North 3rd Ring Road, Chaoyang District, Beijing 100029, China.
Department of Biochemistry and Biomedical Sciences, McMaster University, 1280 Main Street West, Hamilton, ON, L8S 4K1, Canada.
Molecules. 2017 Feb 28;22(3):383. doi: 10.3390/molecules22030383.
Geniposide is a key iridoid glycoside from Gardenia jasminoides fructus widely used in traditional Chinese herbal medicine. However, detection of this small molecule represents a significant challenge mostly due to the lack of specific molecular recognition elements. In this study, we have performed in vitro selection experiments to isolate DNA aptamers that can specifically bind geniposide. Using a stringent selection procedure, we have isolated DNA aptamers that can distinguish geniposide from genipin and glucose, two structural analogs of geniposide. Two top aptamers exhibit low micromolar binding affinity towards geniposide, but show significantly reduced affinity to genipin and glucose. These aptamers have the potential to be further developed into analytical tools for the detection of geniposide.
栀子苷是一种主要来源于中药栀子果实的重要环烯醚萜苷。然而，检测这种小分子存在重大挑战，主要原因是缺乏特异性分子识别元件。在本研究中，我们进行了体外筛选实验，以分离能够特异性结合栀子苷的DNA适配体。通过严格的筛选程序，我们分离出了能够区分栀子苷与其两种结构类似物京尼平及葡萄糖的DNA适配体。两种最佳适配体对栀子苷表现出低微摩尔级的结合亲和力，但对京尼平和葡萄糖的亲和力显著降低。这些适配体有潜力进一步开发成为检测栀子苷的分析工具。
