Costa F, Barbisan F, Assmann C E, Araújo N K F, de Oliveira A R, Signori J P, Rogalski F, Bonadiman B, Fernandes M S, da Cruz I B M
Programa de Pós-Graduação em Farmacologia,Universidade Federal de Santa Maria,Santa Maria-RS,Brazil.
Programa de Pós-Graduação em Bioquímica Toxicológica,Universidade Federal de Santa Maria,Santa Maria-RS,Brazil.
Zygote. 2017 Apr;25(2):111-119. doi: 10.1017/S0967199416000307. Epub 2017 Mar 7.
Previous investigations suggested that elevated cell-free DNA (cfDNA) can indicate non-healthy states. However, the potential association between cfDNA seminal plasma levels and fertility sperm parameters has not yet been determined. Therefore, the present study evaluated the association between seminal cfDNA levels and sperm fertility criteria to determine the use of seminal cfDNA quantification. An in vivo protocol quantified cfDNA levels of semen samples obtained from 163 male patients using fluorescent PicoGreen dye staining. To confirm if semen cfDNA quantification is realistic, an in vitro complementary test was performed using three or four semen samples. The fresh sperm samples were exposed to paraquat that generates high levels of superoxide anion causing oxidative stress and cell mortality. The results showed significant association between dsDNA levels and several sperm fertility parameters, such as low viability and alterations of motility and morphology. The in vitro analysis confirmed the association between dsDNA levels and sperm viability. Together, these results suggest that dsDNA levels could be an important biomarker to test sperm fertility.
先前的研究表明，游离细胞DNA（cfDNA）水平升高可能表明身体处于非健康状态。然而，cfDNA在精浆中的水平与精子生育参数之间的潜在关联尚未确定。因此，本研究评估了精浆cfDNA水平与精子生育标准之间的关联，以确定精浆cfDNA定量分析的用途。采用体内实验方案，使用荧光PicoGreen染料染色对163名男性患者的精液样本中的cfDNA水平进行定量分析。为了确认精液cfDNA定量分析是否切实可行，使用三到四个精液样本进行了体外补充试验。将新鲜精子样本暴露于百草枯中，百草枯会产生高水平的超氧阴离子，导致氧化应激和细胞死亡。结果显示，双链DNA（dsDNA）水平与多个精子生育参数之间存在显著关联，如活力低下以及运动能力和形态的改变。体外分析证实了dsDNA水平与精子活力之间的关联。综上所述，这些结果表明，dsDNA水平可能成为检测精子生育能力的一项指标。
