D'Souza Roshan, Pinto Naina Adren, Higgins Paul G, Hwang Insik, Yong Dongeun, Choi Jongrak, Lee Kyoungwon, Chong Yunsop
Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, South Korea.
Brain Korea 21 PLUS Project for Medical Science, Yonsei University, Seoul, South Korea.
Antimicrob Agents Chemother. 2017 Apr 24;61(5). doi: 10.1128/AAC.02676-16. Print 2017 May.
We identified the carbapenemase gene , a variant of , from a clinical isolate of for the first time. OXA-499 shared 93.1% amino acid identity with OXA-143, and the gene was located on the chromosome. By cloning the OXA-499-encoding gene into the pWH1266 vector and transforming it into susceptible spp., we were able to show that OXA-499 confers resistance to carbapenems.
我们首次从一株临床分离株中鉴定出碳青霉烯酶基因，即的一个变体。OXA-499与OXA-143的氨基酸同一性为93.1%，该基因位于染色体上。通过将编码OXA-499的基因克隆到pWH1266载体中并将其转化到敏感的 spp. 中，我们能够证明OXA-499赋予对碳青霉烯类的抗性。
