Hansen S H, Helboe P, Thomsen M
Department of Organic Chemistry, Royal Danish School of Pharmacy, Copenhagen, Denmark.
J Chromatogr. 1987 Nov 13;409:71-80. doi: 10.1016/s0021-9673(01)86784-4.
The chromatographic behaviour in the reversed-phase mode of various drug substances, which are nitrogen-containing bases, has been investigated. Three different types of column materials were used, ordinary chemically bonded ODS-silica, deactivated ODS-silica specifically designed for the separation of bases and bare silica dynamically modified by adding long-chain quaternary ammonium compounds to the eluent. Various brands were tested for all three types of column materials. When using ODS-silica as the column material, considerable tailing of peaks and prolonged retention was observed. Also the deactivated columns exhibited tailing problems, but less pronounced. For both types of bonded-phase material it was possible to reduce the tailing considerably by the addition of an anti-tailing agent to the eluent. On bare silica used in the dynamic modification mode the tailing was almost negligible.
对各种含氮碱性药物在反相模式下的色谱行为进行了研究。使用了三种不同类型的柱材料，普通化学键合的ODS硅胶、专门为分离碱性物质设计的去活化ODS硅胶以及通过向洗脱液中添加长链季铵化合物进行动态改性的裸硅胶。对所有三种类型的柱材料测试了不同品牌。使用ODS硅胶作为柱材料时，观察到峰明显拖尾且保留时间延长。去活化柱也存在拖尾问题，但不太明显。对于这两种键合相材料，通过向洗脱液中添加抗拖尾剂可以显著减少拖尾。在动态改性模式下使用的裸硅胶上，拖尾几乎可以忽略不计。
