Dobrev Petre I, Hoyerová Klára, Petrášek Jan
Laboratory of Hormonal Regulations in Plants, Institute of Experimental Botany, CAS, Rozvojová 263, 165 02, Prague 6, Czech Republic.
Department of Experimental Plant Biology, Faculty of Science, Charles University, Viničná 5, 128 44, Prague 2, Czech Republic.
Methods Mol Biol. 2017;1569:31-39. doi: 10.1007/978-1-4939-6831-2_2.
Parallel determination of auxin and cytokinin levels within plant organs and tissues represents an invaluable tool for studies of their physiological effects and mutual interactions. Thanks to their different chemical structures, auxins, cytokinins and their metabolites are often determined separately, using specialized procedures of sample purification, extraction, and quantification. However, recent progress in the sensitivity of analytical methods of liquid chromatography coupled to mass spectrometry (LC-MS) allows parallel analysis of multiple compounds. Here we describe a method that is based on single step purification protocol followed by LC-MS separation and detection for parallel analysis of auxins, cytokinins and their metabolites in various plant tissues and cell cultures.
同时测定植物器官和组织中的生长素和细胞分裂素水平，是研究它们的生理效应和相互作用的一项宝贵工具。由于生长素、细胞分裂素及其代谢物的化学结构不同，通常使用专门的样品纯化、提取和定量程序分别进行测定。然而，液相色谱-质谱联用（LC-MS）分析方法灵敏度的最新进展使得能够对多种化合物进行同时分析。本文描述了一种方法，该方法基于单步纯化方案，随后进行LC-MS分离和检测，用于同时分析各种植物组织和细胞培养物中的生长素、细胞分裂素及其代谢物。
