Smith Ewart A, Lewis Ainsely, Morrison Erin N, Molina-Bean Kimberly, Narine Suresh S, Emery R J Neil
Environmental and Life Sciences Graduate Program, Trent University, Peterborough, ON K9J 0G2, Canada.
Department of Biology, Trent University, Peterborough, ON K9J 0G2, Canada.
Metabolites. 2025 Aug 6;15(8):533. doi: 10.3390/metabo15080533.
: Possessing red and white ecotypes, and utilized in traditional Guyanese medicine, ' red ecotype is preferred locally for its purported superior therapeutic efficacy. Although therapeutic metabolites were detected in previously, phytohormones remain largely unexplored, until now. Cytokinins, phytohormones responsible for plant cell division, growth and differentiation, are gaining traction for their therapeutic potential in human health. This study screened and quantified endogenous cytokinins and correlated detected cytokinins with selected secondary metabolites. Liquid chromatography-mass spectrometry was used to acquire phytohormone and metabolite data. Bioinformatics tools were used to assess untargeted metabolomics datasets via statistical and pathway analyses, and chemical groupings of putative metabolites. : In total, 20 of the 35 phytohormones were detected and quantified in both ecotypes, with the red ecotype displaying higher free base and glucoside cytokinin concentrations and exhibited 6.2 times the total CK content when compared to the white ecotype. Pathway analysis revealed flavonoid and monoterpenoid biosynthesis in red and white ecotypes, respectively. Positive correlations between specific cytokinins and alkaloids, and between -Zeatin and isopentenyladenosine riboside with phenolic compounds were observed. These results suggest that the red ecotype's elevated cytokinin levels coupled with flavonoid biosynthesis enrichment support its preference in Guyanese traditional medicine.
具有红色和白色生态型，并被用于圭亚那传统医学，当地更青睐红色生态型，因为其据称具有更高的治疗功效。尽管此前已检测到治疗性代谢物，但植物激素在很大程度上仍未被探索，直到现在。细胞分裂素作为负责植物细胞分裂、生长和分化的植物激素，因其在人类健康方面的治疗潜力而受到越来越多的关注。本研究筛选并定量了内源性细胞分裂素，并将检测到的细胞分裂素与选定的次生代谢物进行了关联。使用液相色谱 - 质谱联用技术获取植物激素和代谢物数据。利用生物信息学工具通过统计和通路分析以及推定代谢物的化学分组来评估非靶向代谢组学数据集。总共在两种生态型中检测并定量了35种植物激素中的20种，红色生态型显示出更高的游离碱和葡萄糖苷细胞分裂素浓度，与白色生态型相比，其总CK含量是白色生态型的6.2倍。通路分析分别揭示了红色和白色生态型中的黄酮类化合物和单萜类化合物的生物合成。观察到特定细胞分裂素与生物碱之间以及玉米素和异戊烯基腺苷核糖苷与酚类化合物之间存在正相关。这些结果表明，红色生态型中细胞分裂素水平的升高以及黄酮类生物合成的富集支持了其在圭亚那传统医学中的偏好。
