Hussain Anwar, Ullah Ihsan, Hasnain Shahida
Department of Botany, Garden Campus, Abdul Wali Khan University, Mardan, 23200, Pakistan.
Department of Environmental Science, Islamic International University, Islamabad, Sector H-10, Islamabad, Pakistan.
Methods Mol Biol. 2017;1569:61-72. doi: 10.1007/978-1-4939-6831-2_4.
Microbial associations with plants are crucial for the survival of both the partners. Beside other ways of establishing such associations, phytohormones enjoy a key role in plant-microbe interactions from initial dialog between the two to the establishment of a viable partnership. Cytokinins (CKs) and IAA are among the five classical groups of phytohormones implicated in plant immune response, early signaling, and deciding the fate of interactions between plant and microbes. Here we describe a method to study modulation of Cks and IAA in plant under the influence of a pathogenic bacterium, Pseudomonas syringae tomato DC3000. A method for inoculating bacteria on host plant and subsequent determination of Cks and IAA through HPLC-ESI-MS/MS is described.
微生物与植物的关联对双方的生存都至关重要。除了建立这种关联的其他方式外，植物激素在植物与微生物的相互作用中起着关键作用，从两者之间的初始对话到建立可行的伙伴关系。细胞分裂素（CKs）和吲哚乙酸（IAA）属于参与植物免疫反应、早期信号传导以及决定植物与微生物相互作用命运的五大经典植物激素类别。在这里，我们描述了一种在致病性细菌丁香假单胞菌番茄致病变种DC3000影响下研究植物中CKs和IAA调节的方法。描述了一种在宿主植物上接种细菌并随后通过高效液相色谱 - 电喷雾串联质谱法（HPLC-ESI-MS/MS）测定CKs和IAA的方法。
