Ludwig-Müller Jutta, Auer Susann, Jülke Sabine, Marschollek Sabine
Institut für Botanik, Technische Universität Dresden, Zellescher Weg 20b, 01062, Dresden, Germany.
Methods Mol Biol. 2017;1569:41-60. doi: 10.1007/978-1-4939-6831-2_3.
The symptoms of the clubroot disease on Brassica species caused by the obligate biotrophic protist Plasmodiophora brassicae relies, among other factors, on the modulation of plant hormones. Signaling, transport as well as biosynthesis and metabolism are key features how the levels of auxins and cytokinins are controlled. We here describe (a) how to inoculate the model plant Arabidopsis thaliana with P. brassicae, (b) qualitative and quantitative methods to evaluate disease severity in auxin and cytokinin mutants,
由专性活体营养型原生生物芸苔根肿菌引起的十字花科植物根肿病症状，除其他因素外，还依赖于植物激素的调节。生长素和细胞分裂素水平的控制方式主要体现在信号传导、运输以及生物合成和代谢等方面。我们在此描述：（a）如何用芸苔根肿菌接种模式植物拟南芥；（b）评估生长素和细胞分裂素突变体中病害严重程度的定性和定量方法。
