调控宿主-病原体界面处植物激素细胞分裂素的水平。

Modulating the Levels of Plant Hormone Cytokinins at the Host-Pathogen Interface.

作者信息

Naseem Muhammad, Shams Shabana, Roitsch Thomas

机构信息

Deptartment of Bioinformatics, Biocenter University of Würzburg Am Hubland Würzburg Germany, Julius-Maximilians-Universität Würzburg, Würzburg, Germany.

Department of Molecular Biology and Genetics, Boğaziçi University, North Campus Kuzey Park Building 310, 34342, Bebek, Instanbul, Turkey.

出版信息

Methods Mol Biol. 2017;1569:141-150. doi: 10.1007/978-1-4939-6831-2_11.

DOI:10.1007/978-1-4939-6831-2_11

PMID:28265994

Abstract

Cytokinins are adenine and non-adenine derived heterogeneous class of regulatory molecules that participate in almost every aspect of plant biology. They also affect plant defense responses as well as help microbial pathogens to establish pathogenesis. The functional approaches that ensure desired and subtle modulations in the levels of plant cytokinins are highly instrumental in assessing their functions in plant immunity. Here, we describe a detailed working protocol regarding the enhanced production of cytokinins from plants that harbor isopentenyltransferase (IPT) enzyme gene under the control of 4xJERE (jasmonic acid and elicitor-responsive element) pathogen-inducible promoter. Our devised expression system is a context-dependent solution when it comes to investigating host-pathogen interactions under the modulated conditions of plant cytokinins.

摘要

细胞分裂素是一类由腺嘌呤和非腺嘌呤衍生而来的异质性调节分子，几乎参与植物生物学的各个方面。它们还影响植物防御反应，并帮助微生物病原体建立致病机制。确保植物细胞分裂素水平得到理想且精细调节的功能方法，对于评估其在植物免疫中的功能非常有帮助。在此，我们描述了一个详细的工作方案，该方案涉及在4xJERE（茉莉酸和激发子响应元件）病原体诱导型启动子控制下，从携带异戊烯基转移酶（IPT）酶基因的植物中增强细胞分裂素的产生。当涉及到在植物细胞分裂素调节条件下研究宿主-病原体相互作用时，我们设计的表达系统是一种依具体情况而定的解决方案。