分析植物-线虫相互作用过程中的细胞分裂素反应
Analyzing Cytokinin Responses During Plant-Nematode Interactions.
作者信息
Grundler Florian M W, Siddique Shahid
机构信息
Department of Molecular Phytomedicine, Rheinische Friedrich-Wilhelms-University of Bonn, Karlrobert-Kreiten-Strasse 13, 53115, Bonn, Germany.
出版信息
Methods Mol Biol. 2017;1569:151-158. doi: 10.1007/978-1-4939-6831-2_12.
Cyst nematodes are obligate biotrophs that induce the formation of a hypertrophied and hypermetabolic syncytial-feeding site in roots of the host plants. Cytokinin signaling is activated at the site of infection and contributes significantly to the formation of syncytium. Here, we describe a protocol for visualizing cytokinin signaling activation in Arabidopsis upon infection with cyst nematode Heterodera schachtii using promoter reporter lines, TCSn:GFP and ARR5:GUS.
胞囊线虫是专性活体营养型生物，可在寄主植物根部诱导形成肥大且代谢活跃的合胞体取食位点。细胞分裂素信号传导在感染部位被激活，并对合胞体的形成有显著贡献。在此，我们描述了一种使用启动子报告株系TCSn:GFP和ARR5:GUS来观察拟南芥在被胞囊线虫甜菜孢囊线虫感染后细胞分裂素信号传导激活情况的方法。
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