Daniel M D, Letvin N L, Sehgal P K, Hunsmann G, Schmidt D K, King N W, Desrosiers R C
New England Regional Primate Research Center, Harvard Medical School, Southborough, Massachusetts 01772.
J Gen Virol. 1987 Dec;68 ( Pt 12):3183-9. doi: 10.1099/0022-1317-68-12-3183.
Juvenile rhesus macaques 6 to 18 months of age were experimentally infected by intravenous inoculation with the simian immunodeficiency virus (SIV), the T cell-tropic retrovirus of monkeys related to the human acquired immunodeficiency syndrome (AIDS) virus HIV. The SIV used for inoculation was grown either in normal human peripheral blood lymphocytes in the presence of interleukin 2 or in the human tumour cell line HUT-78. Eight of the macaques died 129 to 352 days post-inoculation with a variety of clinical and pathological findings paralleling those of AIDS in humans. However eight other animals became persistently infected for prolonged periods; these eight macaques remained alive at 537 and 820 days post-inoculation despite persistent lymphadenopathy and our continued ability to isolate SIV. The ability of these monkeys to survive infection correlated directly with the strength of their antibody response to SIV. Infection was also established in macaques using approximately 100 tissue culture infectious doses of HUT-78-grown SIV. There was no correlation between the dose of virus inoculum and either the strength of the antibody response or clinical outcome. These results demonstrate that SIV infection of macaques can be used not only to study acute AIDS but also to mimic the long-term persistent infection seen in carriers of HIV.
6至18个月大的幼年恒河猴通过静脉注射接种猿猴免疫缺陷病毒（SIV）进行实验性感染，SIV是一种与人类获得性免疫缺陷综合征（AIDS）病毒HIV相关的猴T细胞嗜性逆转录病毒。用于接种的SIV要么在白细胞介素2存在的情况下在正常人外周血淋巴细胞中培养，要么在人肿瘤细胞系HUT - 78中培养。其中8只猕猴在接种后129至352天死亡，出现了与人类艾滋病相似的各种临床和病理表现。然而，另外8只动物长期持续感染；这8只猕猴在接种后537天和820天时仍然存活，尽管存在持续性淋巴结病，并且我们仍能分离出SIV。这些猴子在感染后存活的能力与其对SIV抗体反应的强度直接相关。使用大约100个组织培养感染剂量的在HUT - 78中培养的SIV也在猕猴中建立了感染。病毒接种剂量与抗体反应强度或临床结果之间均无相关性。这些结果表明，猕猴的SIV感染不仅可用于研究急性艾滋病，还可模拟HIV携带者中出现的长期持续感染。
