Department of Dermatology, Tokyo Medical University, Tokyo, Japan.
Department of Radiology, Institute of Medical Science, University of Tokyo, Tokyo, Japan.
Exp Dermatol. 2017 Jul;26(7):619-621. doi: 10.1111/exd.13327. Epub 2017 Apr 21.
Immune checkpoint inhibitors and kinase inhibitors have improved prognosis of malignant melanoma (MM) patients. However, these therapies cannot completely overcome the metastasis of MM. Thus, development of new therapy against metastasis should be required. A first step towards this goal, the aim of this study, is to establish a model of pulmonary metastasis from primary cutaneous MM and a monitoring system. B16-F10, a murine melanoma cell line, was subcutaneously injected into the pinna of mice. The pinna was excised when the lesion was detected. A metastatic nodule on T2-weighted imaging was detected 4 weeks after resection of the pinna. Lung metastases were observed in 37.5% (6/16) of the specimens. We established a novel murine model of the high pulmonary metastasis of MM. The MRI was useful for observations of the growth of the metastatic lesions in the lungs without dissection.
免疫检查点抑制剂和激酶抑制剂改善了恶性黑素瘤（MM）患者的预后。然而，这些治疗方法并不能完全克服 MM 的转移。因此，需要开发针对转移的新疗法。为此，本研究旨在建立一个原发性皮肤 MM 肺转移模型和监测系统。将 B16-F10，一种鼠黑素瘤细胞系，皮下注射到小鼠的耳郭。当发现病变时，切除耳郭。耳郭切除后 4 周，在 T2 加权成像上检测到一个转移结节。在 37.5%（16 个标本中的 6 个）的标本中观察到肺转移。我们建立了一种新的 MM 高肺转移的鼠模型。MRI 可用于在不解剖的情况下观察肺部转移病变的生长。
