Arunorat Jirapat, Charoenvisal Nataya, Woonwong Yonlayong, Kedkovid Roongtham, Jittimanee Supattra, Sitthicharoenchai Panchan, Kesdangsakonwut Sawang, Poolperm Pariwat, Thanawongnuwech Roongroje
Department of Pathology, Faculty of Veterinary Science, Chulalongkorn University, Henri-Dunant Rd, Bangkok 10330, Thailand.
Department of Medicine, Faculty of Veterinary Science, Chulalongkorn University, Henri-Dunant Rd, Bangkok 10330, Thailand.
Res Vet Sci. 2017 Oct;114:6-11. doi: 10.1016/j.rvsc.2017.02.022. Epub 2017 Feb 28.
Since the pandemic H1N1 emergence in 2009 (pdmH1N1), many reassortant pdmH1N1 viruses emerged and found circulating in the pig population worldwide. Currently, commercial human subunit vaccines are used commonly to prevent the influenza symptom based on the WHO recommendation. In case of current reassortant swine influenza viruses transmitting from pigs to humans, the efficacy of current human influenza vaccines is of interest. In this study, influenza A negative pigs were vaccinated with selected commercial human subunit vaccines and challenged with rH3N2. All sera were tested with both HI and SN assays using four representative viruses from the surveillance data in 2012 (enH1N1, pdmH1N1, rH1N2 and rH3N2). The results showed no significant differences in clinical signs and macroscopic and microscopic findings among groups. However, all pig sera from vaccinated groups had protective HI titers to the enH1N1, pdmH1N1 and rH1N2 at 21DPV onward and had protective SN titers only to pdmH1N1and rH1N2 at 21DPV onward. SN test results appeared more specific than those of HI tests. All tested sera had no cross-reactivity against the rH3N2. Both studied human subunit vaccines failed to protect and to stop viral shedding with no evidence of serological reaction against rH3N2. SIV surveillance is essential for monitoring a novel SIV emergence potentially for zoonosis.
自2009年大流行性H1N1（pdmH1N1）出现以来，许多重配pdmH1N1病毒出现并在全球猪群中传播。目前，根据世界卫生组织的建议，常用商业性人亚单位疫苗来预防流感症状。万一当前的重配猪流感病毒从猪传播给人，当前人用流感疫苗的效力就成为人们关注的问题。在本研究中，对甲型流感阴性猪接种选定的商业性人亚单位疫苗，并用rH3N2进行攻毒。使用2012年监测数据中的四种代表性病毒（enH1N1、pdmH1N1、rH1N2和rH3N2），通过血凝抑制（HI）试验和血清中和（SN）试验对所有血清进行检测。结果显示，各组之间在临床症状、大体和微观检查结果方面无显著差异。然而，接种疫苗组的所有猪血清在攻毒后21天（DPV）及以后对enH1N1、pdmH1N1和rH1N2具有保护性HI效价，且在攻毒后21天及以后仅对pdmH1N1和rH1N2具有保护性SN效价。SN试验结果似乎比HI试验结果更具特异性。所有检测血清对rH3N2均无交叉反应。两种研究的人亚单位疫苗均未能提供保护作用，也未能阻止病毒排出，且没有针对rH3N2的血清学反应证据。猪流感病毒监测对于监测可能导致人畜共患病的新型猪流感病毒出现至关重要。
