Kirsten Karina Schreiner, Canova Raíssa, Soveral Lucas de Figueiredo, Friedrich Maria Tereza, Frandoloso Rafael, Kreutz Luiz Carlos
Laboratório de Microbiologia e Imunologia Avançada - Prédio G3, UPF, FAMV, Brazil.
Laboratório de Cromatografia - UPF, FAMV, Brazil.
Fish Shellfish Immunol. 2017 May;64:78-83. doi: 10.1016/j.fsi.2017.03.006. Epub 2017 Mar 3.
The effect of atrazine (ATZ) and its metabolites on aquatic vertebrate species has been a matter of concern to researchers and environmentalist. In this study we exposed head kidney monocytes to sublethal concentrations of atrazine (1 and 10 μg/ml), corresponding to 1% and 10% of the LC, to evaluate the expression of immune-related genes central to immune stimulation. The mRNA levels of TNF-α, Mieloperoxidase and Mx genes were significantly reduced following 24 h exposure to both concentrations of ATZ. The mRNA levels of iRAK4 were reduced only at the higher ATZ concentration and the mRNA levels of IL-1β were not affected. The results reported here support our previous findings on the immunosuppressive effect of ATZ indicating its potential to interfere with the expression of immune-related genes, and strengthen the need to regulate ATZ usage aiming to preserve animal and human health.
阿特拉津（ATZ）及其代谢产物对水生脊椎动物物种的影响一直是研究人员和环保主义者关注的问题。在本研究中，我们将头肾单核细胞暴露于亚致死浓度的阿特拉津（1和10μg/ml），分别相当于半数致死浓度（LC）的1%和10%，以评估免疫刺激核心的免疫相关基因的表达。在暴露于两种浓度的ATZ 24小时后，肿瘤坏死因子-α（TNF-α）、髓过氧化物酶和Mx基因的mRNA水平显著降低。白细胞介素-1受体相关激酶4（iRAK4）的mRNA水平仅在较高的ATZ浓度下降低，而白细胞介素-1β（IL-1β）的mRNA水平未受影响。此处报告的结果支持了我们之前关于ATZ免疫抑制作用的发现，表明其有可能干扰免疫相关基因的表达，并强化了为保护动物和人类健康而规范ATZ使用的必要性。
