Akpaka Patrick Eberechi, Kissoon Shivnarine, Wilson Clyde, Jayaratne Padman, Smith Ashley, Golding George R
Dept. of Paraclinical Sciences, The University of the West Indies, St. Augustine, Trinidad & Tobago.
Dept. of Pathology and Microbiology, King Edward VII Memorial Hospital, Hamilton, Bermuda.
PLoS One. 2017 Mar 7;12(3):e0171317. doi: 10.1371/journal.pone.0171317. eCollection 2017.
Molecular characteristics of vancomycin resistant enterococci isolates from Bermuda Island is currently unknown. This study was conducted to investigate phenotypic and genotypic characteristics of VRE isolates from Bermuda Island using the chromogenic agar, E-tests, polymerase chain reaction (PCR), pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). Eighteen E. faecium isolates were completely analyzed and were all resistant to vancomycin, susceptible to linezolid and quinupristin/dalfopristin, positive for vanA and esp genes. The MLST analysis confirmed most isolates were of the sequence types linked to clonal complex 17 (CC17) that is widely associated with outbreaks in hospitals. Infection control measures, antibiotic stewardship, and surveillance activities will continue to be a priority in hospital on the Island.
目前尚不清楚来自百慕大岛的耐万古霉素肠球菌分离株的分子特征。本研究旨在使用显色琼脂、E试验、聚合酶链反应（PCR）、脉冲场凝胶电泳（PFGE）和多位点序列分型（MLST）来调查来自百慕大岛的耐万古霉素肠球菌分离株的表型和基因型特征。对18株屎肠球菌分离株进行了全面分析，这些分离株均对万古霉素耐药，对利奈唑胺和奎奴普丁/达福普汀敏感，vanA和esp基因呈阳性。MLST分析证实，大多数分离株属于与克隆复合体17（CC17）相关的序列类型，而CC17与医院爆发密切相关。感染控制措施、抗生素管理和监测活动仍将是该岛医院的优先事项。
