Carson James P, Rennie Monique Y, Danilchik Michael, Thornburg Kent, Rugonyi Sandra
Annu Int Conf IEEE Eng Med Biol Soc. 2016 Aug;2016:1236-1239. doi: 10.1109/EMBC.2016.7590929.
Subdivision-based image registration has previously been applied to co-localize digital information extracted from rigid structures in biological specimens, such as the brain. Here, we describe and demonstrate the creation and application of a two-dimensional subdivision-based atlas representing a dynamic structure: the outflow tract of the developing chicken heart. The atlas is designed to segment three different anatomical layers of the outflow tract, and is demonstrated on the characterization of collagen XIV in both control and induced abnormal flow specimens. Abnormal blood flow in the embryonic developing heart can lead to congenital heart disease. Comparing local cellular and sub-cellular changes that are caused by abnormal flow can assist in understanding the molecular pathways involved in maladaptations of the heart and congenital defects. This study demonstrates the approach and potential for more extensive applications of the subdivision-based atlas for the embryonic chicken heart.
基于细分的图像配准先前已应用于对从生物标本（如大脑）的刚性结构中提取的数字信息进行共定位。在此，我们描述并展示了一个基于二维细分的图谱的创建和应用，该图谱代表一种动态结构：发育中的鸡心脏流出道。该图谱旨在对流出道的三个不同解剖层进行分割，并在对照和诱导异常血流标本中对胶原蛋白XIV的特征进行了展示。胚胎发育心脏中的异常血流可导致先天性心脏病。比较由异常血流引起的局部细胞和亚细胞变化有助于理解心脏适应不良和先天性缺陷所涉及的分子途径。本研究展示了基于细分的图谱在胚胎鸡心脏中更广泛应用的方法和潜力。
