Mercier-Bodard C, Radanyi C, Roux C, Groyer M T, Robel P, Dadoune J P, Petra P H, Jolly D J, Baulieu E E
Lab. Hormones, Inserm U 33, Bicêtre, France.
J Steroid Biochem. 1987;27(1-3):297-307. doi: 10.1016/0022-4731(87)90321-9.
The cellular distribution of human Sex Steroid Binding Plasma Protein (h-SBP) was studied in human cells and tissues by indirect immunofluorescence. h-SBP was detected in the cytoplasm of hepatocytes, of prostate and epididymis epithelial cells and in endometrium. Sexual and non-sexual skin, intestine epithelium, striated muscle and some rodent organs were not labelled. The intracellular localization of h-SBP indicate that h-SBP could be taken up from the extracellular compartment or synthesized in situ in sex steroid target organs, where it may play a role in hormone uptake. The hormonal regulation of h-SBP secretion by a human hepatoma cell line, H5A, showed that tri-iodothyronine was more potent than estradiol or tamoxifen, which acted as estrogen agonist, in increasing secreted h-SBP and the combined effect of both thyroid and estrogen hormones resulted in an additive stimulation of h-SBP secretion. As shown by Northern blot analysis, oligonucleotides synthesized from the known sequence of h-SBP hybridized with a RNA of approximately 2 kb which was more represented in H5A cells than in normal human liver, and was increased 2-3 times after hormonal stimulation of the cells. The presence of a poly(A+)RNA coding for h-SBP in the human liver indicated the hepatic synthesis of this protein.
通过间接免疫荧光法研究了人类性甾体结合血浆蛋白（h-SBP）在人类细胞和组织中的细胞分布。在肝细胞、前列腺和附睾上皮细胞的细胞质以及子宫内膜中检测到了h-SBP。性皮肤和非性皮肤、肠上皮、横纹肌以及一些啮齿动物器官均未被标记。h-SBP的细胞内定位表明，h-SBP可能从细胞外区室摄取，或在性甾体靶器官中就地合成，在那里它可能在激素摄取中发挥作用。人肝癌细胞系H5A对h-SBP分泌的激素调节表明，三碘甲状腺原氨酸在增加h-SBP分泌方面比作为雌激素激动剂的雌二醇或他莫昔芬更有效，甲状腺激素和雌激素的联合作用导致对h-SBP分泌的累加刺激。如Northern印迹分析所示，从h-SBP已知序列合成的寡核苷酸与一条约2 kb的RNA杂交，该RNA在H5A细胞中的含量比在正常人类肝脏中更高，并且在对细胞进行激素刺激后增加了2至3倍。人肝脏中存在编码h-SBP的多聚腺苷酸RNA（poly(A+)RNA）表明该蛋白是在肝脏中合成的。
