Luppa P, Oettrich K, Schwab I, Langmandel U, Neumeier D
Institute for Clinical Chemistry, Klinikum Grosshadern, University of Munich, FRG.
Acta Endocrinol (Copenh). 1989 Dec;121(6):791-6. doi: 10.1530/acta.0.1210791.
The human hepatoma cell line HEP G2 was investigated in an indirect immunofluorescence study for localization of the sex hormone-binding globulin. Cells were grown directly to confluency on the slides used for the immunocytochemical staining. A fine granular cytoplasmatic fluorescence pattern revealed the presence of sex hormone-binding globulin. Chang-liver cells used as controls, incubated under the same conditions, did not fluoresce. The synthesis of the hormone-binding protein in HEP G2 cells, which could be stimulated by L-thyroxine in the culture medium, was monitored by either indirect immunofluorescence or by immunoradiometric determination of sex hormone-binding globulin in the supernatant. These studies demonstrate that immunofluorescence techniques are able to detect sex hormone-binding globulin in human hepatoma cells.
在一项间接免疫荧光研究中，对人肝癌细胞系HEP G2进行了检测，以确定性激素结合球蛋白的定位。细胞直接在用于免疫细胞化学染色的载玻片上生长至汇合。精细的颗粒状细胞质荧光模式显示存在性激素结合球蛋白。作为对照的Chang肝细胞，在相同条件下孵育，未发出荧光。通过间接免疫荧光或通过免疫放射测定培养基上清液中的性激素结合球蛋白，监测HEP G2细胞中可被培养基中的L-甲状腺素刺激的激素结合蛋白的合成。这些研究表明，免疫荧光技术能够检测人肝癌细胞中的性激素结合球蛋白。
