Beato M, Arnemann J, Chalepakis G, Slater E, Willmann T
Institut für Molekularbiologie und Tumorforschung der Philipps-Universität, Marburg, Federal Republic of Germany.
J Steroid Biochem. 1987;27(1-3):9-14. doi: 10.1016/0022-4731(87)90288-3.
The location, orientation, and structure of the hormone regulatory elements (HRE) in nine hormonally modulated genes is described. Based on analysis of the contact points between the glucocorticoid receptor (GR) and the DNA double helix within the HREs, a model for the interaction is proposed in which a dimer of the receptor in head-to-head orientation binds to the inverted symmetry element of the HRE. The relationship between the regulatory elements for glucocorticoids and progesterone in the long terminal repeat region (LTR) of mouse mammary tumor virus (MMTV), and in the promoter region of the chicken lysozyme gene, indicates that the recognition mechanism for both receptors is similar but not identical. Curiously, the hormone ligand is not an absolute requirement for the GR to bind its HRE, though it influences the kinetics of the interaction. Other possible functions of the hormone in vivo are discussed, as well as the molecular mechanism responsible for transcriptional regulation after receptor binding to the HRE.
本文描述了九个激素调节基因中激素调节元件（HRE）的位置、方向和结构。基于对糖皮质激素受体（GR）与HRE内DNA双螺旋之间接触点的分析，提出了一种相互作用模型，即受体以头对头方向形成的二聚体与HRE的反向对称元件结合。小鼠乳腺肿瘤病毒（MMTV）长末端重复序列（LTR）以及鸡溶菌酶基因启动子区域中糖皮质激素和孕酮调节元件之间的关系表明，两种受体的识别机制相似但不完全相同。奇怪的是，激素配体并非GR结合其HRE的绝对必要条件，尽管它会影响相互作用的动力学。文中还讨论了激素在体内的其他可能功能以及受体与HRE结合后负责转录调控的分子机制。
