Revello M G, Percivalle E, Zavattoni M, Gerna G
Virus Laboratory, Institute of Infectious Diseases, University of Pavia, Italy.
Microbiologica. 1987 Oct;10(4):393-401.
A new capture ELISA kit (ETI-Rubek-M) for rubella IgM antibody determination was compared with a commercial reverse (Rubenostika IgM) and two indirect ELISAs (Rubelisa M and Rubazyme-M). Of the 89 sera from 38 patients with acute rubella infection, 70 gave identical results with the four assays (78% concordance), 74 were in agreement in the two indirect ELISAs, and 83 concorded in the reverse assays (83% and 93% concordance, respectively). On the whole, ETI-Rubek-M and Rubenostika IgM proved equally sensitive; sensitivity of the indirect ELISA kits was lower, Rubazyme-M being slightly more sensitive than Rubelisa M. Specificity of the two reverse assays was 100%, whereas Rubelisa M and Rubazyme-M showed a specificity of 88.8% and 93.3%, respectively. In indirect ELISAs, false positive IgM results were mainly observed in cytomegalovirus IgM-positive sera from patients recovering from a primary cytomegalovirus infection. Two sequential sera from one of these patients with IgM to cytomegalovirus were found to be true positive for rubella-specific IgM antibody with ETI-Rubek-M, Rubelisa M and Rubazyme-M.
将一种用于风疹IgM抗体测定的新型捕获ELISA试剂盒（ETI-Rubek-M）与一种市售的反向ELISA试剂盒（Rubenostika IgM）以及两种间接ELISA试剂盒（Rubelisa M和Rubazyme-M）进行了比较。在来自38例急性风疹感染患者的89份血清中，70份在这四种检测方法中结果相同（一致性为78%），74份在两种间接ELISA试剂盒中结果一致，83份在反向ELISA检测中结果一致（分别为83%和93%的一致性）。总体而言，ETI-Rubek-M和Rubenostika IgM的敏感性相当；间接ELISA试剂盒的敏感性较低，Rubazyme-M比Rubelisa M稍敏感一些。两种反向ELISA检测的特异性均为100%，而Rubelisa M和Rubazyme-M的特异性分别为88.8%和93.3%。在间接ELISA检测中，假阳性IgM结果主要出现在原发性巨细胞病毒感染康复患者的巨细胞病毒IgM阳性血清中。其中一名巨细胞病毒IgM阳性患者的两份连续血清，用ETI-Rubek-M、Rubelisa M和Rubazyme-M检测发现风疹特异性IgM抗体呈真阳性。
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