Fortier B, Nicolas J C, Bricout F
Pathol Biol (Paris). 1983 Sep;31(7):599-600.
An enzyme-linked immunosorbent assay (ELISA) was compared to sucrose gradient centrifugation technique for rubella specific IgM detection of 114 sera. The 28 sera which were rubella specific IgM positive, were also positive by ELISA. Among the 86 sera which were rubella IgM negative by centrifugation, 6 were equivocal and 2 were rubella IgM positive by the ELISA test, without clinical or epidemiological connection. These false results make a necessity of confirming by an immunoglobulin separation technique the ELISA IgM positive sera.
采用酶联免疫吸附测定（ELISA）法与蔗糖梯度离心技术对114份血清进行风疹特异性IgM检测，并比较两种方法的检测结果。28份风疹特异性IgM阳性血清，ELISA检测结果也为阳性。在经离心法检测风疹IgM阴性的86份血清中，有6份结果可疑，2份ELISA检测风疹IgM为阳性，但无临床或流行病学关联。这些假阳性结果表明，对于ELISA IgM阳性血清，有必要采用免疫球蛋白分离技术进行确认。
