Arista S, Pistoia D, Titone L, Ammatuna P
Microbiologica. 1987 Apr;10(2):151-60.
Four commercial kits- the hemagglutination inhibition test Behring (HI-B), the HI Rubenosticon, the latex test Rubalex, the enzyme-linked immunosorbent assay (ELISA) ETI-RUBEK G--and a non-commercial hemolysis test in gel (SRH) were compared for the measurement of rubella IgG antibodies by testing 126 serum samples, submitted for rubella immune status determination. Two commercial ELISAs (ETI-RUBEK M and Rubazyme M) were also evaluated for the detection of rubella IgM antibodies on 64 sera from patients with suspected acute rubella infection. In the assay for rubella specific IgG antibodies, HI-B and ETI-RUBEK G tests exhibited the most sensitivity, whereas Rubenosticon and Rubalex tests showed more specificity. In the IgM evaluations, six false positive results were shown by Rubazyme M, whereas the ETI-RUBEK M kit was sensitive and specific. On the basis of all the determinations, a combination of two tests (HI-B and SRH) appears to be a good simple system to obtain valid indications in serological rubella diagnosis.
通过检测126份用于风疹免疫状态测定的血清样本，对四种商业试剂盒——贝林血凝抑制试验（HI-B）、鲁本诺斯蒂康血凝抑制试验、鲁巴莱克斯乳胶试验、酶联免疫吸附测定（ELISA）ETI-RUBEK G——以及一种非商业凝胶溶血试验（SRH）进行了比较，以测定风疹IgG抗体。还对两种商业ELISA试剂盒（ETI-RUBEK M和鲁巴酶M）进行了评估，用于检测64份疑似急性风疹感染患者血清中的风疹IgM抗体。在风疹特异性IgG抗体检测中，HI-B和ETI-RUBEK G试验表现出最高的灵敏度，而鲁本诺斯蒂康试验和鲁巴莱克斯试验显示出更高的特异性。在IgM评估中，鲁巴酶M出现了6例假阳性结果，而ETI-RUBEK M试剂盒既灵敏又特异。基于所有测定结果，两种试验（HI-B和SRH）的组合似乎是在风疹血清学诊断中获得有效指征的良好简单系统。
