Wang Xinchen, Yang Meiling, Wang Zhiyuan, Zhang Hua, Wang Guofeng, Deng Min, Chen Huanwen, Luo Liping
Jiangxi Key Laboratory for Mass Spectrometry and Instrumentation, East China University of Technology , Nanchang 330013, People's Republic of China.
Nanchang County the First Secondary School in Liantang , Nanchang 330046, People's Republic of China.
J Agric Food Chem. 2017 Mar 22;65(11):2488-2494. doi: 10.1021/acs.jafc.7b00553. Epub 2017 Mar 14.
The molecular fingerprinting of intact fruit samples combined with statistical data analysis can allow the assessment of fruit quality and location of origin. Herein, microwave plasma torch desorption ionization mass spectrometry (MPT-MS) was applied to produce molecular fingerprints for the juice sac and exocarp of navel oranges cultivated in three closely located habitats, and the mass spectrometric fingerprints were differentiated by principal component analysis (PCA). Because of the relatively high temperature and high ionization efficiency of MPT, the volatile aroma compounds and semivolatile chemicals in the navel oranges were sensitively detected and confidently identified by collision induced dissociation (CID). The limit of detection (LOD) of MPT-MS for vanillin was 0.119 μg/L, with the relative standard deviation (RSD, n = 10) of 1.7%. The results showed that MPT-MS could be a powerful analytical platform for the sensitive molecular analysis of fruits at molecular level with high chemical specificity, allowing differentiation between the same sorts grown in neighboring habitats.
完整水果样本的分子指纹图谱结合统计数据分析能够评估水果品质和产地。在此，采用微波等离子体炬解吸电离质谱法（MPT-MS）对生长在三个相邻产地的脐橙汁胞和外果皮进行分子指纹图谱分析，并通过主成分分析（PCA）对质谱指纹图谱进行区分。由于MPT温度较高且电离效率高，脐橙中的挥发性香气化合物和半挥发性化学物质能够通过碰撞诱导解离（CID）被灵敏检测并可靠鉴定。MPT-MS对香草醛的检测限为0.119μg/L，相对标准偏差（RSD，n = 10）为1.7%。结果表明，MPT-MS可作为一个强大的分析平台，在分子水平上对水果进行灵敏的分子分析，具有高化学特异性，能够区分相邻产地种植的同一品种水果。
