Takebe Y, Seiki M, Fujisawa J, Hoy P, Yokota K, Arai K, Yoshida M, Arai N
Department of Molecular Biology, DNAX Research Institute of Molecular and Cellular Biology, Palo Alto, California 94304.
Mol Cell Biol. 1988 Jan;8(1):466-72. doi: 10.1128/mcb.8.1.466-472.1988.
We developed a novel promoter system, designated SR alpha, which is composed of the simian virus 40 (SV40) early promoter and the R segment and part of the U5 sequence (R-U5') of the long terminal repeat of human T-cell leukemia virus type 1. The R-U5' sequence stimulated chloramphenicol acetyltransferase (CAT) gene expression only when placed immediately downstream of the SV40 early promoter in the sense orientation. The SR alpha expression system was 1 or 2 orders of magnitude more active than the SV40 early promoter in a wide variety of cell types, including fibroblasts and lymphoid cells, and was capable of promoting a high level of expression of various lymphokine cDNAs. These features of the SR alpha promoter were incorporated into the pcD-cDNA expression cloning vector originally developed by Okayama and Berg.
我们开发了一种新型启动子系统，命名为SRα，它由猿猴病毒40（SV40）早期启动子以及人类1型T细胞白血病病毒长末端重复序列的R片段和部分U5序列（R-U5'）组成。仅当R-U5'序列以正义方向紧邻SV40早期启动子下游放置时，它才能刺激氯霉素乙酰转移酶（CAT）基因的表达。在包括成纤维细胞和淋巴细胞在内的多种细胞类型中，SRα表达系统的活性比SV40早期启动子高1至2个数量级，并且能够促进各种淋巴因子cDNA的高水平表达。SRα启动子的这些特性被整合到了最初由冈山县和伯格开发的pcD-cDNA表达克隆载体中。
