Shimotohno K, Golde D W, Miwa M, Sugimura T, Chen I S
Proc Natl Acad Sci U S A. 1984 Feb;81(4):1079-83. doi: 10.1073/pnas.81.4.1079.
The nucleotide sequence of the human T-cell leukemia virus type II (HTLV-II) long terminal repeat (LTR) and its surrounding regions were determined. Our results show the following structural features: (i) the LTR is 763 base pairs (bp) in length and consists of 314 +/- 1 bp of region U3, 248 +/- 1 bp of region R, and 201 bp of region U5; (ii) the terminal nucleotides in the LTR form an inverted repeat of T-G.....C-A; (iii) 6-bp direct repeats of cellular sequences flanking the provirus were present; and (iv) the putative functional signals for initiation or termination of viral RNA synthesis were identified. Comparison of the HTLV-II LTR sequence with that previously published for adult T-cell leukemia virus (ATLV; HTLV-I) shows that the LTRs are distinct. Some small regions are conserved between HTLV-II and ATLV, involving sequences important for transcription and a sequence of 21 nucleotides repeated three times in U3. This 21-bp repeat may be important in regulating viral transcription in lymphoid cells.
测定了人类II型T细胞白血病病毒（HTLV-II）长末端重复序列（LTR）及其周边区域的核苷酸序列。我们的结果显示出以下结构特征：（i）LTR长度为763个碱基对（bp），由U3区域的314±1 bp、R区域的248±1 bp和U5区域的201 bp组成；（ii）LTR中的末端核苷酸形成T-G.....C-A的反向重复序列；（iii）前病毒两侧存在细胞序列的6 bp直接重复序列；（iv）鉴定出了病毒RNA合成起始或终止的假定功能信号。将HTLV-II LTR序列与先前发表的成人T细胞白血病病毒（ATLV；HTLV-I）的序列进行比较，结果表明LTR是不同的。HTLV-II和ATLV之间存在一些小的保守区域，包括对转录重要序列以及在U3中重复三次的21个核苷酸序列。这个21 bp的重复序列可能在调节淋巴样细胞中的病毒转录方面很重要。
