Ghasemi Fatemeh, Parvin Parviz, Motlagh Najme Sadat Hosseini, Abachi Shahriar
Physics Department, Amirkabir University of Technology, P.O. Box 15875-4413, Tehran, Iran.
Physics & Astronomy Department, California State University 90032, Los Angeles, California, USA.
Biomed Opt Express. 2017 Jan 3;8(2):512-523. doi: 10.1364/BOE.8.000512. eCollection 2017 Feb 1.
We employ laser induced fluorescence (LIF) spectroscopy to discriminate between normal and cancerous human breast (in-vitro) tissues. LIF signals are usually enhanced by the exogenous agents such as Rhodamine 6G (Rd6G) and Coumarin 7 (C7). Although we observe fluorescence emissions in both fluorophores, Rd6G-stained tissues give notable spectral red shift in practice. The latter is a function of dye concentration embedded in tissues. We find that such red shifts have a strong dependence on the dye concentration in bare, in stained healthy, and in malignant breast tissues, signifying variations in tubular abundances. In fact, the heterogeneity of cancerous tissues is more prominent mainly due to their notable tubular densities- which can provide numerous micro-cavities to house more dye molecules. We show that this can be used to discriminate between the healthy and unhealthy specimens in different biological scaffolds of ordered (healthy) and disordered (cancerous) tissues. It is demonstrated that the quenching process of fluorophore' molecules slows down in the neoplastic tumors according to the micro-partitioning, too.
我们采用激光诱导荧光（LIF）光谱法来区分正常和癌性人体乳腺（体外）组织。LIF信号通常会被诸如罗丹明6G（Rd6G）和香豆素7（C7）等外源性试剂增强。虽然我们在两种荧光团中都观察到了荧光发射，但实际上Rd6G染色的组织会出现明显的光谱红移。后者是组织中嵌入染料浓度的函数。我们发现，这种红移在裸露的、染色的健康乳腺组织和恶性乳腺组织中都强烈依赖于染料浓度，这表明管状丰度存在差异。事实上，癌组织的异质性更为突出，主要是由于其显著的管状密度——这可以提供大量微腔来容纳更多染料分子。我们表明，这可用于区分有序（健康）和无序（癌性）组织的不同生物支架中的健康和不健康标本。结果表明，根据微分配，荧光团分子在肿瘤中的猝灭过程也会减慢。
